Document Detail


Analysis of inositol phosphates in heart tissue using anion-exchange high-performance liquid chromatography.
MedLine Citation:
PMID:  9278239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pathways of release and metabolism of inositol phosphates in intact heart tissue are different from those observed in isolated cardiomyocytes in culture. Thus, it is essential that methods are available for the quantitation of inositol phosphates in intact tissue preparations. This manuscript describes methods which allow the quantitation of inositol phosphates in different heart preparations including isolated atria and intact perfused heart. The availability of such methods should facilitate study of the role of inositol phosphates in cardiac control mechanisms under physiological and pathological conditions.
Authors:
E A Woodcock
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  172     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1997 Jul 
Date Detail:
Created Date:  1997-10-14     Completed Date:  1997-10-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  121-7     Citation Subset:  IM    
Affiliation:
Cellular Biochemistry Laboratory, Baker Medical Research Institute, Prahran, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anion Exchange Resins
Chromatography, High Pressure Liquid / methods*
Chromatography, Ion Exchange
Freeze Drying
Heart Ventricles / chemistry
Humans
Inositol Phosphates / chemistry*,  isolation & purification
Mice
Myocardium / chemistry*
Perfusion
Rats
Trichloroacetic Acid
Chemical
Reg. No./Substance:
0/Anion Exchange Resins; 0/Inositol Phosphates; 76-03-9/Trichloroacetic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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