Document Detail

Analysis of innervation of human mesenteric vessels in non-inflamed and inflamed bowel--a confocal and functional study.
MedLine Citation:
PMID:  18298440     Owner:  NLM     Status:  MEDLINE    
We investigated the distribution and density of perivascular nerves in human mesenteric arteries and veins and their responses to noradrenaline (NA), ATP and neuropeptide Y (NPY) in control (non-inflamed) and inflamed bowel, using confocal microscopy and in vitro pharmacology. The density of innervation at the adventitial-medial border of arteries and within the medial muscle coat of veins was increased in inflammatory bowel disease (IBD). Expression of markers for both sympathetic nerves and sensory-motor nerves was significantly increased in IBD. Calcitonin gene-related peptide-containing sensory-motor nerves were present in control arteries and IBD, but not in control veins. The density of 5-hydroxytryptamine-containing nerves was variable in controls, but consistently increased (three to four times) in IBD. Vasoactive intestinal peptide (VIP) expression increased (doubled) in arteries and veins. Arteries and veins contracted to NA and ATP, but only veins constricted to NPY. ATP contractions were reduced in arteries and veins in IBD, while contractions to NA were only slightly reduced. Neuropeptide Y induced significantly greater (20%) contractions of IBD veins. In summary, the density of sympathetic and sensory-motor innervation of both mesenteric arteries and veins was increased in IBD. Both 5-hydroxytryptamine and VIP immunoreactivity were also increased. The responses of both arteries and veins to ATP, and to a lesser extent NA, were reduced in IBD while responses to NPY were greater in veins. Decreased responses to ATP indicate changes in purinergic-mediated transmission in the pathological state.
D Birch; G E Knight; P B Boulos; G Burnstock
Publication Detail:
Type:  Comparative Study; Journal Article     Date:  2008-02-19
Journal Detail:
Title:  Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society     Volume:  20     ISSN:  1365-2982     ISO Abbreviation:  Neurogastroenterol. Motil.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-16     Completed Date:  2008-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9432572     Medline TA:  Neurogastroenterol Motil     Country:  England    
Other Details:
Languages:  eng     Pagination:  660-70     Citation Subset:  IM    
Autonomic Neuroscience Centre, Royal Free and University College Medical School, Rowland Hill Street, London, UK.
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MeSH Terms
Aged, 80 and over
Digestive System Diseases / pathology,  physiopathology*
Gastrointestinal Tract / blood supply*,  cytology,  innervation*
Inflammatory Bowel Diseases / pathology,  physiopathology*
Mesenteric Arteries / cytology,  innervation*,  physiology
Mesenteric Veins / cytology,  innervation*,  physiology
Mesentery / blood supply,  cytology,  physiology
Microscopy, Confocal / methods
Middle Aged

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