| Analysis of inducible contractile rings suggests a role for protein kinase C in embryonic cytokinesis and wound healing. | |
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MedLine Citation:
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PMID: 1751967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A semi-in vitro system derived from Xenopus oocytes which allows induction of contractile ring (CR) formation and closure is described and exploited to elucidate regulatory and structural features of cytokinesis. The inducible CRs (ICRs) are composed of actin filaments and closure is actin filament-dependent as is cytokinesis in vivo. ICR closure in this system is calcium-dependent and pH-sensitive, as is cytokinesis in permeabilized cells (Cande: Journal of Cell Biology 87:326, 1980). Closure of ICRs proceeds at a rate and with a kinetic pattern similar to embryonic cytokinesis. Collectively, these data demonstrate that this system is a faithful mimic of cytokinesis in vivo. ICR formation and closure is protein kinase C (PKC)-dependent and neomycin-sensitive, indicating that the PKC branch of the polyphosphoinositide pathway regulates formation of the actomyosin ring which is the effector of cytokinesis. Kinetic measurements show that the rate of ICR closure reaches a peak of 4-8 microns/sec. Since the maximum measured velocity of actin filament translocation by vertebrate, non-muscle myosins is 0.04 micron/sec, the later observations support a model in which the CR is segmented, containing multiple sites where filaments overlap in a "sliding filament" fashion. Because the rate decreases after reaching a peak, the results also suggest that the number of overlap sites decrease with time. |
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Authors:
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W M Bement; D G Capco |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Cell motility and the cytoskeleton Volume: 20 ISSN: 0886-1544 ISO Abbreviation: Cell Motil. Cytoskeleton Publication Date: 1991 |
Date Detail:
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Created Date: 1992-01-24 Completed Date: 1992-01-24 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8605339 Medline TA: Cell Motil Cytoskeleton Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 145-57 Citation Subset: IM |
Affiliation:
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Department of Zoology, Arizona State University, Tempe 85287-1501. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkaloids
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pharmacology Animals Calcium / pharmacology Cell Division / drug effects* Cell-Free System Egtazic Acid / pharmacology Female Hydrogen-Ion Concentration Neomycin / pharmacology Oocytes Phorbol Esters / pharmacology Protein Kinase C / antagonists & inhibitors, physiology* Second Messenger Systems / drug effects Sphingosine / pharmacology Staurosporine Wound Healing / drug effects* Xenopus laevis |
| Grant Support | |
ID/Acronym/Agency:
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HD00598/HD/NICHD NIH HHS; HD23686/HD/NICHD NIH HHS; RR05097/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Alkaloids; 0/Phorbol Esters; 123-78-4/Sphingosine; 1404-04-2/Neomycin; 62996-74-1/Staurosporine; 67-42-5/Egtazic Acid; 7440-70-2/Calcium; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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