| Analysis of in vitro transfection by sonoporation using cationic and neutral microbubbles. | |
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MedLine Citation:
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PMID: 20800945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of the study was to examine the role of acoustic power intensity and microbubble and plasmid concentrations on transfection efficiency in HEK-293 cells using a sonoporator with a 1-MHz transducer. A green fluorescent protein (GFP) reporter plasmid was delivered in as much as 80% of treated cells, and expression of the GFP protein was observed in as much as 75% of cells, using a power intensity of 2 W/cm(2) with a 25% duty cycle. In addition, the relative transfection abilities of a lipid noncationic and cationic microbubble platform were investigated. As a positive control, cells were transfected using Lipofectamine reagent. Cell survival and transfection efficiency were inversely proportional to acoustic power and microbubble concentration. Our results further demonstrated that high-efficiency transfection could be achieved, but at the expense of cell loss. Moreover, direct conjugation of plasmid to the microbubble did not appear to significantly enhance transfection efficiency under the examined conditions, although this strategy may be important for targeted transfection in vivo. |
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Authors:
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Jose L Tlaxca; Christopher R Anderson; Alexander L Klibanov; Bryce Lowrey; John A Hossack; J Steven Alexander; Michael B Lawrence; Joshua J Rychak |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Ultrasound in medicine & biology Volume: 36 ISSN: 1879-291X ISO Abbreviation: Ultrasound Med Biol Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-25 Completed Date: 2011-02-28 Revised Date: 2011-11-01 |
Medline Journal Info:
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Nlm Unique ID: 0410553 Medline TA: Ultrasound Med Biol Country: England |
Other Details:
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Languages: eng Pagination: 1907-18 Citation Subset: IM |
Copyright Information:
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Copyright © 2010. Published by Elsevier Inc. |
Affiliation:
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Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cations Cell Line Cell Survival Cells, Cultured Flow Cytometry Gene Expression Regulation Gene Transfer Techniques Green Fluorescent Proteins / genetics* Microbubbles* Microscopy, Fluorescence Plasmids Transducers Transfection Ultrasonics* |
| Grant Support | |
ID/Acronym/Agency:
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1R43DK079406-01/DK/NIDDK NIH HHS; EB002185/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cations; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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