Document Detail


Analysis of in vitro transfection by sonoporation using cationic and neutral microbubbles.
MedLine Citation:
PMID:  20800945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective of the study was to examine the role of acoustic power intensity and microbubble and plasmid concentrations on transfection efficiency in HEK-293 cells using a sonoporator with a 1-MHz transducer. A green fluorescent protein (GFP) reporter plasmid was delivered in as much as 80% of treated cells, and expression of the GFP protein was observed in as much as 75% of cells, using a power intensity of 2 W/cm(2) with a 25% duty cycle. In addition, the relative transfection abilities of a lipid noncationic and cationic microbubble platform were investigated. As a positive control, cells were transfected using Lipofectamine reagent. Cell survival and transfection efficiency were inversely proportional to acoustic power and microbubble concentration. Our results further demonstrated that high-efficiency transfection could be achieved, but at the expense of cell loss. Moreover, direct conjugation of plasmid to the microbubble did not appear to significantly enhance transfection efficiency under the examined conditions, although this strategy may be important for targeted transfection in vivo.
Authors:
Jose L Tlaxca; Christopher R Anderson; Alexander L Klibanov; Bryce Lowrey; John A Hossack; J Steven Alexander; Michael B Lawrence; Joshua J Rychak
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Ultrasound in medicine & biology     Volume:  36     ISSN:  1879-291X     ISO Abbreviation:  Ultrasound Med Biol     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-25     Completed Date:  2011-02-28     Revised Date:  2013-05-28    
Medline Journal Info:
Nlm Unique ID:  0410553     Medline TA:  Ultrasound Med Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1907-18     Citation Subset:  IM    
Copyright Information:
Copyright © 2010. Published by Elsevier Inc.
Affiliation:
Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA.
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MeSH Terms
Descriptor/Qualifier:
Cations
Cell Line
Cell Survival
Cells, Cultured
Flow Cytometry
Gene Expression Regulation
Gene Transfer Techniques
Green Fluorescent Proteins / genetics*
Microbubbles*
Microscopy, Fluorescence
Plasmids
Transducers
Transfection
Ultrasonics*
Grant Support
ID/Acronym/Agency:
1R43DK079406-01/DK/NIDDK NIH HHS; EB002185/EB/NIBIB NIH HHS
Chemical
Reg. No./Substance:
0/Cations; 147336-22-9/Green Fluorescent Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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