| Analysis of hydroxyproline isomers and hydroxylysine by reversed-phase HPLC and mass spectrometry. | |
| | |
MedLine Citation:
|
PMID: 17085085 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Collagens, the most abundant mammalian proteins, contain a high content of hydroxylated amino acids, such as, 3- and 4-cis-/trans-hydroxyproline (Hyp) and 5-hydroxylysine (Hyl). Whereas the global content of 4-Hyp was studied by amino acid analysis, no technique to determine all five hydroxyamino acids simultaneously in collagens has been reported. Here, we report the separation of all five hydroxyamino acids as well as two Hyp epimers from all other proteinogenic amino acids after derivatization with N(2)-(5-fluoro-2,4-dinitrophenyl)-l-valine amide (l-FDVA) by RPC-UV-ESI-MS. The general applicability of this method is shown for three Hyp-containing peptides as well as collagen type I. |
| | |
Authors:
|
Tobias Langrock; Natividad García-Villar; Ralf Hoffmann |
Related Documents
:
|
23265985 - Experimental comparison of the effects of locally administered zoledronic acid and alen... 1148885 - Mineralization of elastin extracted from human aortic tissues. 20024875 - Chemical peels: what's new and what isn't new but still works well. 9816495 - Endogenous amino acids in budgerigars (melopsittacus undulatus). 41405 - Blood acid-base equilibrium in experimental acidosis of the rumen in sheep. 20532275 - Unusual fluorescein prototropism within aqueous acidic 1-butyl-3-methylimidazolium tetr... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-07 |
Journal Detail:
|
Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Volume: 847 ISSN: 1570-0232 ISO Abbreviation: J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. Publication Date: 2007 Mar |
Date Detail:
|
Created Date: 2007-03-06 Completed Date: 2007-05-21 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101139554 Medline TA: J Chromatogr B Analyt Technol Biomed Life Sci Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 282-8 Citation Subset: IM |
Affiliation:
|
Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine (BBZ), Faculty of Chemistry and Mineralogy, University of Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Chromatography, High Pressure Liquid / methods* Collagen / chemistry Hydroxylysine / analysis*, chemistry Hydroxyproline / analysis*, chemistry Isomerism Rats Spectrometry, Mass, Electrospray Ionization / methods* |
| Chemical | |
Reg. No./Substance:
|
28902-93-4/Hydroxylysine; 51-35-4/Hydroxyproline; 9007-34-5/Collagen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Determination by liquid chromatography-mass spectrometry of clomiphene isomers in the plasma of pati...
Next Document: Proteomic analysis of isolated membrane fractions from superinvasive cancer cells.