Document Detail

Analysis of hydroxyproline isomers and hydroxylysine by reversed-phase HPLC and mass spectrometry.
MedLine Citation:
PMID:  17085085     Owner:  NLM     Status:  MEDLINE    
Collagens, the most abundant mammalian proteins, contain a high content of hydroxylated amino acids, such as, 3- and 4-cis-/trans-hydroxyproline (Hyp) and 5-hydroxylysine (Hyl). Whereas the global content of 4-Hyp was studied by amino acid analysis, no technique to determine all five hydroxyamino acids simultaneously in collagens has been reported. Here, we report the separation of all five hydroxyamino acids as well as two Hyp epimers from all other proteinogenic amino acids after derivatization with N(2)-(5-fluoro-2,4-dinitrophenyl)-l-valine amide (l-FDVA) by RPC-UV-ESI-MS. The general applicability of this method is shown for three Hyp-containing peptides as well as collagen type I.
Tobias Langrock; Natividad García-Villar; Ralf Hoffmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-11-07
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  847     ISSN:  1570-0232     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-03-06     Completed Date:  2007-05-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  282-8     Citation Subset:  IM    
Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine (BBZ), Faculty of Chemistry and Mineralogy, University of Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany.
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MeSH Terms
Amino Acid Sequence
Chromatography, High Pressure Liquid / methods*
Collagen / chemistry
Hydroxylysine / analysis*,  chemistry
Hydroxyproline / analysis*,  chemistry
Spectrometry, Mass, Electrospray Ionization / methods*
Reg. No./Substance:
28902-93-4/Hydroxylysine; 51-35-4/Hydroxyproline; 9007-34-5/Collagen

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