Document Detail


Analysis of human chorionic gonadotropin (hCG): application of routine immunological methods for initial testing and confirmation analysis in doping control.
MedLine Citation:
PMID:  23303576     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human chorionic gonadotropin (hCG) is dimeric glycoprotein produced by placenta in pregnancy and also in low levels by pituitary gland. The main clinical use for exogenous hCG-administration is typically linked to infertility. The desired effect of hCG misuse in sport is due to the enhancement of testicular production of testosterone. Therefore, hCG is listed by the World Anti-Doping Agency (WADA) as a prohibited substance in male athletes and according to the recently published WADA guideline urinary concentrations of hCG > 5 IU/L may be an indicator of doping. In this study two independent immunoassays were used to implement the new WADA guideline. The assay for initial testing (Siemens Immulite 2000 XPi hCG assay) recognizes various hCG variants (e.g. hCG and β-core fragment of hCG) whereas the confirmatory assay (PerkinElmer DELFIA Xpress hCG) is sensitive to intact and nicked hCG only. Both assays showed adequate sensitivity and were proven fit-for-purpose in routine doping control. Population-based distribution of the assays was in good agreement with results of earlier studies and supported well the current threshold of 5 IU/L.
Authors:
Tiia Kuuranne; Liisa Ahola; Christel Pussinen; Antti Leinonen
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2013-01-10
Journal Detail:
Title:  Drug testing and analysis     Volume:  5     ISSN:  1942-7611     ISO Abbreviation:  Drug Test Anal     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-08-15     Completed Date:  2014-03-05     Revised Date:  2014-04-29    
Medline Journal Info:
Nlm Unique ID:  101483449     Medline TA:  Drug Test Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  614-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 John Wiley & Sons, Ltd.
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MeSH Terms
Descriptor/Qualifier:
Chorionic Gonadotropin / urine*
Chorionic Gonadotropin, beta Subunit, Human / urine
Doping in Sports
Humans
Immunoassay / methods*
Male
Peptide Fragments / urine
Reproducibility of Results
Sensitivity and Specificity
Substance Abuse Detection / methods*
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Chorionic Gonadotropin, beta Subunit, Human; 0/Peptide Fragments; 0/urinary gonadotropin fragment
Comments/Corrections
Comment In:
Drug Test Anal. 2014 Mar;6(3):301-2   [PMID:  24151058 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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