Document Detail


Analysis of human choriogonadotropin core 2 o-glycan isoforms.
MedLine Citation:
PMID:  12850278     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Measurements of human choriogonadotropin (hCG) isoforms containing core 2 o-glycans may be useful for diagnosis of Down Syndrome pregnancies and trophoblastic disease. As shown here, this isoform is also present in pituitary extracts, early pregnancy urine, and urine of postmenopausal women. Although, measurements of hCG isoforms may be useful in several clinical settings, this remains to be determined due to the lack of suitable standards and the difficulties of comparing data obtained in different laboratories. Here, we report that monoclonal antibodies B152 and CTP104 recognize the third (Ser132) and fourth (Ser138) o-glycans, respectively, in the carboxyterminal portion of the hCG beta-subunit. The proximity of these sites prevents B152 and CTP104 from binding simultaneously to isoforms containing core 2 o-glycans. Unlike B152, which binds only the core 2 isoform, CTP104 recognizes both glycan moieties. By measuring hCG with CTP104 in the presence and absence of B152, one can quantify both isoforms using the same readily available standard.
Authors:
S Birken; Oksana Yershova; Rebecca V Myers; Michael P Bernard; William Moyle
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  204     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-09     Completed Date:  2004-04-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  21-30     Citation Subset:  IM    
Affiliation:
Department of Ob/Gyn of Columbia University College of Physicians and Surgeons, BB1608, 630W, 168th St., New York, NY 10032, USA. sb18@columbia.edu
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / diagnostic use
Antibody Specificity
Chorionic Gonadotropin / immunology,  urine*
Chorionic Gonadotropin, beta Subunit, Human / immunology,  urine
Down Syndrome / diagnosis
Epitopes
Female
Humans
Polysaccharides / immunology*,  urine
Pregnancy
Protein Isoforms / immunology,  urine*
Trophoblastic Neoplasms / diagnosis
Grant Support
ID/Acronym/Agency:
HD14907/HD/NICHD NIH HHS; HD38547/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Chorionic Gonadotropin; 0/Chorionic Gonadotropin, beta Subunit, Human; 0/Epitopes; 0/Polysaccharides; 0/Protein Isoforms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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