Document Detail


Analysis of human cerebrospinal fluid by capillary electrophoresis with laser-induced fluorescence detection.
MedLine Citation:
PMID:  7978320     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Capillary electrophoresis with laser-induced fluorescence detection is used to analyze 10 microL samples of human cerebrospinal fluid. Primary amine-containing compounds in untreated cerebrospinal fluid are labeled with 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde prior to analysis, producing fluorescent isoindoles. Electropherograms containing approximately 50 peaks are obtained in less than 35 min from cerebrospinal fluid samples. Ten peaks in the electropherograms have been identified and quantitated: arginine, glutamine, threonine, valine, gamma-amino-n-butyric acid, serine, alanine, glycine, glutamic acid, and aspartic acid. Detection limits for these 10 amino acids range from 0.29 nM for gamma-amino-n-butyric acid to 100 nM for threonine, and separation efficiencies as high as 190,000 theoretical plates are obtained for these analytes. Electropherograms of cerebrospinal fluid samples from patients with Alzheimer's disease and from children with different neurological disorders are compared to those of healthy controls. Differences in individual amino acid levels are observed between the patient groups, and these differences appear to be disease and age related. These results indicate that analysis of cerebrospinal fluid by capillary electrophoresis will be useful as a selective, rapid, and sensitive tool for both diagnosis of central nervous system disorders and for study of the mechanisms of these disorders.
Authors:
J Bergquist; S D Gilman; A G Ewing; R Ekman
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  66     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-12-22     Completed Date:  1994-12-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3512-8     Citation Subset:  IM    
Affiliation:
Department of Clinical Neuroscience, Göteborg University, Mölndal Hospital, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Amino Acid Sequence
Amino Acids / cerebrospinal fluid
Benzoates
Cerebrospinal Fluid / chemistry*
Child
Electrophoresis / methods
Female
Fluorescence
Fluorescent Dyes
Humans
Lasers
Male
Middle Aged
Molecular Sequence Data
Nervous System Diseases / cerebrospinal fluid
Quinolines
Reference Values
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Benzoates; 0/Fluorescent Dyes; 0/Quinolines; 131124-59-9/3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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