Document Detail

Analysis of host-cell responses by immunoblotting, ELISA, and real-time PCR.
MedLine Citation:
PMID:  22328386     Owner:  NLM     Status:  MEDLINE    
Intracellular responses to external pathogens/stimuli are crucial to the host's response to infection. The methods used to analyse these responses fall into many categories. Activation of proteins as part of a signal cascade can be screened for using conventional immunoblotting techniques or immunoprecipitation to identify the presence of modified proteins or protein complexes. Transcription factor activity can be screened for by EMSA or ELISAs to identify DNA binding of these factors. Finally, expression of activated genes can be quantified using real-time PCR methods. Here, we will show how to perform these assays and discuss the relative merits of each.
David L Moyes; Julian R Naglik
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  845     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-02-13     Completed Date:  2012-06-11     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  345-60     Citation Subset:  IM    
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MeSH Terms
Cells / immunology*
Cells, Cultured
Enzyme-Linked Immunosorbent Assay / methods*
Host-Pathogen Interactions*
Immunoblotting / methods*
Real-Time Polymerase Chain Reaction / methods*
Grant Support
MR/J008303/1//Medical Research Council

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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