Document Detail


Analysis of hop acids and their oxidized derivatives and iso-alpha-acids in beer by capillary electrophoresis-electrospray ionization mass spectrometry.
MedLine Citation:
PMID:  16848524     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigates the applicability of on-line coupling of capillary electrophoresis with electrospray ionization tandem mass spectrometry (CZE-ESI-MS) for the separation and characterization of alpha- and beta-acids and oxidized hop acids from crude extracts of different hop varieties. CZE-ESI-MS with negative-ion electrospray ionization proved to be a suitable technique for the determination of these types of natural compounds and their oxidized derivatives. The CZE parameters (pH, concentration, and buffer type) and ESI-MS parameters (nature and flow rate of the sheath liquid, nebulizer pressure, drying gas flow rate, temperature, and compound stability) were optimized. The optimized method provides the potential for a fast qualitative determination of hop acids and their oxidation compounds. The method was also applied to the determination of iso-alpha-acids in beer.
Authors:
Rocío García-Villalba; Sonia Cortacero-Ramírez; Antonio Segura-Carretero; José Antonio Martín-Lagos Contreras; Alberto Fernández-Gutiérrez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  54     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-19     Completed Date:  2006-08-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5400-9     Citation Subset:  IM    
Affiliation:
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, c/ Fuentenueva s/n, 18071 Granada, Spain.
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MeSH Terms
Descriptor/Qualifier:
Beer / analysis*
Cyclohexenes
Electrophoresis, Capillary*
Humulus / chemistry*
Hydrogen-Ion Concentration
Spectrometry, Mass, Electrospray Ionization*
Terpenes / analysis*
Chemical
Reg. No./Substance:
0/Cyclohexenes; 0/Terpenes; 469-02-3/humulon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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