| Analysis of homogeneous populations of anterior pituitary folliculostellate cells by laser capture microdissection and reverse transcription-polymerase chain reaction. | |
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MedLine Citation:
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PMID: 11316732 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pituitary folliculostellate (FS) cells are usually located between the secretory cells in the anterior pituitary, and they produce many peptides that exert a paracrine effect on hormone-producing pituitary cells. Previous approaches have been unsuccessful in obtaining homogeneous populations of FS cells. We used a combination of immunostaining with S100 protein followed by laser capture microdissection (Immuno-LCM) to obtain purified populations of rat FS cells. These cells were analyzed along with a mouse FS cell line (TtT/GF) by RT-PCR for gene expression. RT-PCR analyses showed that both FS cell populations expressed the mRNAs for glial fibrillary acidic protein, S100 protein, transforming growth factor-beta1 (TGFbeta1), TGFbeta receptor, interleukin-6, leptin, leptin receptor, pituitary adenylate cyclase-activating polypeptide (PACAP), and PACAP receptors. Both FS cell populations were negative for PRL, GH, and POMC, supporting the homogeneity of the rat FS cell population. TGFbeta1, but not PACAP-38, treatment stimulated cell proliferation in both FS cell populations. TGFbeta1 increased leptin, but not interleukin-6, mRNA expression in rat FS cells. However, TGFbeta1 inhibited leptin RNA expression in the TtT/GF cell line, as shown by RT-PCR and Northern blot analysis. These results indicate that 1) homogeneous populations of FS cells can be prepared by Immuno-LCM; 2) TGFbeta1 stimulates the proliferation of normal rat FS cells and the TtT/GF cell line; and 3) the effects of TGFbeta1 to stimulate leptin mRNA expression in rat FS cells but inhibit leptin mRNA expression in TtT/GF cells probably reflect alterations in signal transduction in the TtT/GF cell line. |
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Authors:
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L Jin; I Tsumanuma; K H Ruebel; J M Bayliss; R V Lloyd |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 142 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-04-24 Completed Date: 2001-05-17 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 1703-9 Citation Subset: AIM; IM |
Affiliation:
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Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Division / drug effects Cells, Cultured Dissection Female Immunohistochemistry Lasers Leptin / genetics Neuropeptides / pharmacology Pituitary Adenylate Cyclase-Activating Polypeptide Pituitary Gland, Anterior / cytology* RNA, Messenger / analysis Rats Rats, Inbred WF Reverse Transcriptase Polymerase Chain Reaction* S100 Proteins / analysis Transforming Growth Factor beta / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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CA-37231/CA/NCI NIH HHS; CA-90249/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adcyap1 protein, rat; 0/Leptin; 0/Neuropeptides; 0/Pituitary Adenylate Cyclase-Activating Polypeptide; 0/RNA, Messenger; 0/S100 Proteins; 0/Transforming Growth Factor beta |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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