Document Detail


Analysis of histamine and N tau-methylhistamine in plasma by gas chromatography-negative ion-chemical ionization mass spectrometry.
MedLine Citation:
PMID:  2751105     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Current methods of quantitation of histamine and its major metabolite N tau-methylhistamine are inaccurate and insensitive to the very low concentrations that exist in plasma samples. Therefore, an accurate and sensitive method for quantification in plasma has been developed using the stable isotope dilution assay with negative ion-chemical ionization mass spectrometry. For histamine, after the addition of [2H4]histamine to 2 ml of plasma, the plasma sample is deproteinized, extracted into butanol, back extracted into HCl, derivatized to the pentafluorobenzyl derivative (CH2C6F5)3-histamine, purified on silica gel columns, and then quantified with negative ion-chemical ionization mass spectrometry by selected ion monitoring of the ratio of ions m/z 430/434. For N tau-methylhistamine, after the addition of N tau-[2H3]methylhistamine to 2 ml of plasma, the plasma sample is deproteinized, extracted into butanol, back extracted into HCl, derivatized to the heptafluorobutyryl derivative (C3F7CO2)2-N tau-methylhistamine, purified on silica gel columns, and then quantified with negative ion-chemical ionization mass spectrometry by selected ion monitoring of the ratio of ions m/z 497/500. The precision of the histamine assay is 3.1% and the accuracy is 95.5 +/- 2.5% while the precision of the N tau-methylhistamine assay is 1.9% and the accuracy is 106.8 +/- 1.9%. The lower limits of sensitivity are 1 pg for histamine and 6 pg for N tau-methylhistamine injected on column. Using the assay in three normal human volunteers, plasma concentrations of histamine were 130, 92, and 85 pg/ml, and of N tau-methylhistamine were 229, 228, and 216 pg/ml. This assay provides a very sensitive and accurate method of quantitation of histamine and N tau-methylhistamine in plasma samples.
Authors:
N A Payne; J A Zirrolli; J G Gerber
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  178     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1989 May 
Date Detail:
Created Date:  1989-08-25     Completed Date:  1989-08-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  414-20     Citation Subset:  IM    
Affiliation:
Department of Medicine and Pharmacology, University of Colorado Health Sciences Center, Denver 80262.
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MeSH Terms
Descriptor/Qualifier:
Animals
Dogs
Gas Chromatography-Mass Spectrometry / methods
Histamine / blood*
Humans
Methylhistamines / blood*
Grant Support
ID/Acronym/Agency:
HL 21308/HL/NHLBI NIH HHS; RR 01152/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Methylhistamines; 51-45-6/Histamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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