Document Detail


Analysis of hexosaminitol-containing disaccharide alditols from rat brain glycoproteins and gangliosides as O-trimethylsilyl derivatives by gas chromatography mass spectrometry.
MedLine Citation:
PMID:  912029     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The O-trimethylsilyl derivatives of five reference disaccharide alditols composed of a N-acetylhexosaminitol substituted at C-3, C-4 or C-6 with a hexose moiety were studied by gas chromatography mass spectrometry. The data were used in the determination of the linkage in disaccharide alditols derived from rat brain glycoproteins and gangliosides. The O-glycosidically linked carbohydrate units of brain glycoproteins contained two oligosaccharides, alpha-galactosyl-(1 leads to 3)-N-acetylgalactosaminitol and beta-galactosyl-(1 leads to 3)-N-acetylgalactosaminitol, whereas only the latter was obtained from brain gangliosides after partial acid hydrolysis and reduction.
Authors:
J Finne; I Mononen; J Kärkkäinen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biomedical mass spectrometry     Volume:  4     ISSN:  0306-042X     ISO Abbreviation:  Biomed. Mass Spectrom.     Publication Date:  1977 Oct 
Date Detail:
Created Date:  1977-12-29     Completed Date:  1977-12-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0430246     Medline TA:  Biomed Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  281-3     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain Chemistry*
Disaccharides / analysis*
Gangliosides*
Gas Chromatography-Mass Spectrometry / methods
Glycoproteins*
Hexosamines / analysis*
Rats
Sugar Alcohols / analysis*
Trimethylsilyl Compounds
Chemical
Reg. No./Substance:
0/Disaccharides; 0/Gangliosides; 0/Glycoproteins; 0/Hexosamines; 0/Sugar Alcohols; 0/Trimethylsilyl Compounds

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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