Document Detail


Analysis of glycosaminoglycans of flow sorted cells: incorporation of [35S]sulfate and [3H]glucosamine into glycosaminoglycans of B16-F10 cells during the cell cycle.
MedLine Citation:
PMID:  7172938     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The incorporation of [35S]sulfate and [3H] glucosamine into cetyl pyridinium chloride (CPC) precipitable glycosaminoglycans was determined in B16-F10 cultured cells sorted with respect to DNA content. Incorporation into surface material was measured indirectly as the difference between the radioactivity of control and trypsin treated cells. Approximately 80% of the total cellular [35S] sulfate labeled CPC precipitable material, but only 5% of that labeled by [3H]glucosamine, was removed by this mild trypsin treatment. Incorporation of [35S]sulfate into the trypsin removable surface material increased progressively from G1 to S to F2 + M in both long-term (48 hours) and short-term (1 hour) labeled cells, while the ratio of surface to total incorporated [35S]sulfate remained relatively constant. Incorporation of [35S]sulfate into total cellular glycosaminoglycans in long- and short-term labeled cells increased as cells progressed from G1 to S to G2 + M; the incorporation of [3H]glucosamine into CPC precipitable material also increased progressively from G1 to S to G2 + M in long-term labeled cells but was greater during S phase relative to G1 or G2 + M in short-term labeled cells. The degree of sulfation of glycosaminoglycans as represented by the ratio of [35S]sulfate to [3H]glucosamine of double labeled cells was relatively constant in long-term labeled cells but was increased during the G1 and G2 + M phases of short-term labeled cells. Comparison of the degree of sulfation of short-term with long-term labeled cells suggests that highly sulfated glycosaminoglycans may be turned over more rapidly during G1 and G2 + M phases of the cell cycle.
Authors:
O C Blair; D E Burger; A C Sartorelli
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytometry     Volume:  3     ISSN:  0196-4763     ISO Abbreviation:  Cytometry     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1983-02-14     Completed Date:  1983-02-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8102328     Medline TA:  Cytometry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  166-71     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Separation
DNA / analysis
Flow Cytometry
Glucosamine / metabolism*
Glycosaminoglycans / biosynthesis*
Interphase*
Kinetics
Melanoma
Mice
Mitosis*
Sulfates / metabolism*
Grant Support
ID/Acronym/Agency:
CA-02817/CA/NCI NIH HHS; CA-16359/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 0/Sulfates; 3416-24-8/Glucosamine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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