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Analysis of giant cell tumour of bone cells for Noonan syndrome/Cherubism-related mutations.
MedLine Citation:
PMID:  22725657     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
J Oral Pathol Med (2012) Background:  Giant cell tumour of bone (GCTB) is an osteolytic tumour which contains numerous osteoclast-like giant cells and a proliferation of mononuclear stromal cells (MSC). Giant cell-rich osteolytic lesions can also develop in the jaw bones in Noonan syndrome, a cherubism-like developmental abnormality that is transmitted in an autosomal dominant fashion, often because of mutation in the PTPN11 or BRAF genes. Methods:  We screened GCTBs for mutations in PTPN11 and BRAF to determine whether GCTBs develop through alterations of genes involved in Noonan syndrome. MSC were isolated from 10 GCTBs. Results:  Chromosome banding analysis of these cells revealed telomeric associations (tas) in 7 of the 10 cases. Thus, the cultured cells expressed a cytogenetic abnormality typically found in short-term cultures from GCTBs. Sequencing of DNA extracted from the seven GCTB-derived MSC cultures displaying tas did not identify any mutation in PTPN11 or in exons 9-15 of BRAF. Conclusion:  Our findings indicate that the molecular pathways involved in GCTB development are different from those causing Noonan syndrome. The method for isolating and culturing GCTB stromal cells described in this study generated a population of MSC that contained tas, indicating that it is useful for obtaining stromal cells from GCTB and other giant cell-rich lesions, such as giant cell reparative granuloma, for genetic and other studies.
Authors:
Linda Moskovszky; Bernadine Idowu; Richard Taylor; Fredrik Mertens; Nicholas Athanasou; Adrienne Flanagan
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-22
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  -     ISSN:  1600-0714     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Affiliation:
Semmelweis University 1st Department of Pathology and Experimental Cancer Research, Budapest, Hungary Institute of Orthopaedics and Musculoskeletal Science, University College London, Stanmore, Middlesex, UK Histopathology Department, Royal National Orthopaedic Hospital NHS Trust, Stanmore, UK Department of Pathology, Nuffield Orthopaedic Centre, Oxford, UK Department of Clinical Genetics, Lund University Hospital, Lund, Sweden Cancer Institute, University College Hospital, London, UK.
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