| Analysis of genome plasticity in pathogenic and commensal Escherichia coli isolates by use of DNA arrays. | |
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MedLine Citation:
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PMID: 12618447 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Genomes of prokaryotes differ significantly in size and DNA composition. Escherichia coli is considered a model organism to analyze the processes involved in bacterial genome evolution, as the species comprises numerous pathogenic and commensal variants. Pathogenic and nonpathogenic E. coli strains differ in the presence and absence of additional DNA elements contributing to specific virulence traits and also in the presence and absence of additional genetic information. To analyze the genetic diversity of pathogenic and commensal E. coli isolates, a whole-genome approach was applied. Using DNA arrays, the presence of all translatable open reading frames (ORFs) of nonpathogenic E. coli K-12 strain MG1655 was investigated in 26 E. coli isolates, including various extraintestinal and intestinal pathogenic E. coli isolates, 3 pathogenicity island deletion mutants, and commensal and laboratory strains. Additionally, the presence of virulence-associated genes of E. coli was determined using a DNA "pathoarray" developed in our laboratory. The frequency and distributional pattern of genomic variations vary widely in different E. coli strains. Up to 10% of the E. coli K-12-specific ORFs were not detectable in the genomes of the different strains. DNA sequences described for extraintestinal or intestinal pathogenic E. coli are more frequently detectable in isolates of the same origin than in other pathotypes. Several genes coding for virulence or fitness factors are also present in commensal E. coli isolates. Based on these results, the conserved E. coli core genome is estimated to consist of at least 3,100 translatable ORFs. The absence of K-12-specific ORFs was detectable in all chromosomal regions. These data demonstrate the great genome heterogeneity and genetic diversity among E. coli strains and underline the fact that both the acquisition and deletion of DNA elements are important processes involved in the evolution of prokaryotes. |
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Authors:
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Ulrich Dobrindt; Franziska Agerer; Kai Michaelis; Andreas Janka; Carmen Buchrieser; Martin Samuelson; Catharina Svanborg; Gerhard Gottschalk; Helge Karch; Jörg Hacker |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of bacteriology Volume: 185 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-03-05 Completed Date: 2003-03-31 Revised Date: 2013-04-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: United States |
Other Details:
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Languages: eng Pagination: 1831-40 Citation Subset: IM |
Affiliation:
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Institut für Molekulare Infektionsbiologie der Universität Würzburg, 97070 Würzburg, Germany. ulrich.dobrindt@mail.uni-wuerzburg.de |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AJ496193 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Electrophoresis, Gel, Pulsed-Field Escherichia coli / genetics, pathogenicity* Escherichia coli Infections / microbiology Escherichia coli Proteins / genetics Evolution, Molecular* Genetic Variation* Genome, Bacterial* Humans Molecular Sequence Data Oligonucleotide Array Sequence Analysis / methods* Open Reading Frames / genetics Polymerase Chain Reaction Sequence Analysis, DNA Virulence |
| Chemical | |
Reg. No./Substance:
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0/Escherichia coli Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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