Document Detail

Analysis of genetic diversity at the DQA loci in African cattle: evidence for a BoLA-DQA3 locus.
MedLine Citation:
PMID:  9211751     Owner:  NLM     Status:  MEDLINE    
We describe the development of a polymerase chain reaction (PCR)-based approach for analysis of genetic diversity at the DQA loci in African Bos indicus and Bos taurus cattle. This approach, equally effective in European and Asian cattle breeds, detects the presence or absence of DQA1 and most duplicated DQA2 genes. Nucleotide and predicted amino acid sequence analysis of the highly polymorphic second exons, in addition to analysis of the locus-specific and relatively non-polymorphic transmembrane, cytoplasmic, and 3-prime untranslated regions, has provided evidence for considerable diversity between each of the duplicated DQA2 genes. Therefore, we propose the designation BoLA-DQA3 for the previously unpublished alleles at the second DQA2 locus. Fourteen distinct PCR restriction fragment length polymorphism (RFLP) patterns, each identifying families of alleles at three DQA loci, can be distinguished. Nucleotide sequence analysis of new PCR-RFLP patterns from 193 Kenyan Boran, Ethiopian Arsi (B. indicus), and Guinean N'Dama (B. taurus) cattle identified 13 DQA1 alleles within eight major allelic families, five DQA2 alleles within a single allelic family, and seven DQA3 alleles within three major allelic families.
K T Ballingall; A Luyai; D J McKeever
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunogenetics     Volume:  46     ISSN:  0093-7711     ISO Abbreviation:  Immunogenetics     Publication Date:  1997  
Date Detail:
Created Date:  1997-08-13     Completed Date:  1997-08-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0420404     Medline TA:  Immunogenetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  237-44     Citation Subset:  IM    
International Livestock Research Institute (ILRI), Box 30709, Nairobi, Kenya.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Y10560;  Y10561;  Y10562;  Y10563;  Y10564;  Y10565;  Z79502;  Z79503;  Z79504;  Z79505;  Z79506;  Z79507;  Z79508;  Z79509;  Z79510;  Z79511;  Z79512;  Z79513;  Z79514;  Z79515;  Z79516;  Z79517;  Z79518;  Z79519;  Z79520;  Z79521;  Z79522;  Z79523;  Z79524;  Z79525
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cattle / genetics*
Genes, MHC Class II / genetics*
Histocompatibility Antigens Class II / genetics*
Molecular Sequence Data
Polymerase Chain Reaction / methods
Polymorphism, Restriction Fragment Length
Sequence Alignment
Sequence Homology, Nucleic Acid
Terminology as Topic
Reg. No./Substance:
0/Histocompatibility Antigens Class II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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