| Analysis of gamma-hydroxybutyric acid, DL-lactic acid, glycolic acid, ethylene glycol and other glycols in body fluids by a direct injection gas chromatography-mass spectrometry assay for wide use. | |
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MedLine Citation:
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PMID: 15576294 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Analysis of blood of severely intoxicated patients always requires prompt investigation. Diagnosis of intoxication with ethylene glycol, gamma-hydroxybutyric acid or D-lactic acid takes hours, since several different procedures are required. Rapid derivatization of the common hydroxyl function may resolve this analytical problem. Here we describe a fast method for the simultaneous measurement of ethylene glycol, glycolic acid, gamma-hydroxybutyric acid and racemic lactic acid. Only 20 microl of serum, plasma or urine are required for immediate derivatization at 70 degrees C with 750 microl of bis-N,O-trimethylsilyl trifluoroacetamide after adding 20 microl of internal standard solution (1,3-propylene glycol) and 20 microl of the catalyst dimethylformamide. After centrifugation an aliquot is transferred to a gas chromatographic system and analyzed with electron-impact mass spectrometry in selective ion monitoring mode. The derivatized acids and ethylene glycol are well separated and detected with a limit of detection ranging from 0.12 mg/l for ethylene glycol to 0.95 mg/l for gamma-hydroxybutyric acid, while the limit of quantification ranged from 0.4 mg/l for ethylene glycol to 3.15 mg/l for gamma-hydroxybutyric acid. The method is linear from 0.5 to 1800 mg/l blood for ethylene glycol, from 0.7 to 1200 mg/l for lactic acid, from 1.2 to 1800 mg/l for glycolic acid, and from 3.2 to 200 mg/l for gamma-hydroxybutyric acid, with analytical recoveries, accuracy, day-to-day and within-day precision well within the required limits. Total analysis time with one calibrator was 30 min, derivatization time included. This method is very suitable for emergency toxicology, since several toxic substances can be quantified simultaneously in a fast and sensitive manner. |
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Authors:
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Paul Van Hee; Hugo Neels; Mireille De Doncker; Nicolas Vrydags; Katinka Schatteman; Wim Uyttenbroeck; Nicole Hamers; Dirk Himpe; Willy Lambert |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Clinical chemistry and laboratory medicine : CCLM / FESCC Volume: 42 ISSN: 1434-6621 ISO Abbreviation: Clin. Chem. Lab. Med. Publication Date: 2004 |
Date Detail:
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Created Date: 2004-12-03 Completed Date: 2005-04-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9806306 Medline TA: Clin Chem Lab Med Country: Germany |
Other Details:
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Languages: eng Pagination: 1341-5 Citation Subset: IM |
Affiliation:
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Laboratory of Biochemistry and Toxicology, Ziekenhuis Netwerk Antwerpen Stuivenberg, Antwerp, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography Ethylene Glycol / analysis* Gas Chromatography-Mass Spectrometry / methods* Glycolates / analysis* Glycols / chemistry Humans Hydroxybutyrates / analysis* Ions Kinetics Lactic Acid / analysis* Mass Spectrometry / methods* Sensitivity and Specificity Time Factors Trimethylsilyl Compounds / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Glycolates; 0/Glycols; 0/Hydroxybutyrates; 0/Ions; 0/Trimethylsilyl Compounds; 107-21-1/Ethylene Glycol; 21149-38-2/N,N-bis(trimethylsilyl)-2,2,2-trifluoroacetamide; 50-21-5/Lactic Acid; 591-81-1/4-hydroxybutyric acid; 79-14-1/glycolic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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