| Analysis of free hemoglobin level and hemoglobin peptides from human puerperal uterine secretions. | |
| | |
MedLine Citation:
|
PMID: 16697945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: Hemocidins are a novel class of antibacterial peptides generated proteolytically from hemoglobin. These peptides play a particularly important role in maintaining vaginal homeostasis during menstrual bleeding. To investigate the hemoglobin fragmentation process during the last stages of pregnancy, we examined uterine secretion (lochia) samples from a group of 22 healthy women who underwent cesarean delivery at term. METHODS: Patients were divided into three groups: (1) the elective cesarean deliveries without symptoms of spontaneous labor, (2) the nonelective cesarean deliveries with spontaneous beginning of labor, and (3) the nonelective cesarean deliveries during advanced labor. The samples were subjected to chromatographic estimation of free hemoglobin and peptides. In three representative patients the identification of all lochial peptides was performed. RESULTS: All samples contained a significant amount of free hemoglobin and its level increased with labor progression. The presence of peptide fractions was also detected in most lochia samples. They were confirmed to be human hemoglobin fragments, almost identical to the recently described bactericidal hemocidins from menstrual discharge. The level of peptides also increased during labor. The subgroup with advanced labor demonstrated the highest amount of hemocidins. CONCLUSIONS: The presented results prove that proteolysis of free hemoglobin in the female upper reproductive tract begins together with the clinical symptoms of normal labor. We speculate that cesarean delivery affects molecular mechanisms involved in antibacterial hemocidins generation and, in effect, might be responsible for the increased risk of gynecologic infections in cesarean deliveries. |
| | |
Authors:
|
Pawel Mak; Lukasz Wicherek; Piotr Suder; Adam Dubin; Tomasz Banas; Irena Kaim; Marek Klimek |
Related Documents
:
|
2800975 - Comparative clinical study of induction and emergence time in sevoflurane and enflurane... 934565 - Studies of human pregnancy. i. immunoglobulins attached to the trophoblast. 9849705 - Obstetric outcome of elderly low-risk nulliparae. 10687835 - Uterine rupture in a multiparous woman during labor induction with oral misoprostol. 1137335 - Hepatotis of pregnancy. a clinical study of 107 patients. 12734035 - Psychopharmacologic treatment of depression during pregnancy. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of the Society for Gynecologic Investigation Volume: 13 ISSN: 1556-7117 ISO Abbreviation: J. Soc. Gynecol. Investig. Publication Date: 2006 May |
Date Detail:
|
Created Date: 2006-05-15 Completed Date: 2006-06-12 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 9433806 Medline TA: J Soc Gynecol Investig Country: United States |
Other Details:
|
Languages: eng Pagination: 285-91 Citation Subset: IM |
Affiliation:
|
Department of Analytical Biochemistry, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland. makp@mol.uj.edu.pl |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Anti-Bacterial Agents
/
analysis* Bacterial Infections / etiology Cesarean Section / adverse effects Chromatography Electrophoresis Female Hemoglobins / analysis*, metabolism* Homeostasis Humans Immunity, Innate Mass Spectrometry Peptide Fragments / analysis* Postpartum Period Pregnancy Risk Factors Uterus / secretion* Vagina / chemistry, microbiology |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Hemoglobins; 0/Peptide Fragments |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Association of fetal choroid plexus cysts with trisomy 18 in a population previously screened by nuc...
Next Document: The depolarized expression of the alpha-6 integrin subunit in the endometria of women with endometri...