| Analysis of flagellin perception mediated by flg22 receptor OsFLS2 in rice. | |
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MedLine Citation:
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PMID: 18986259 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Plants have sensitive perception systems that recognize various pathogen-derived molecules. We previously reported that rice detects flagellin from a rice-incompatible strain of gram-negative phytopathogenic bacterium, Acidovorax avenae, which induces subsequent immune responses involving cell death. The mechanism of flagellin perception in rice, however, has remained obscure. In this study, we found that flg22, a peptide derived from the flagellin N-terminus, induced weak immune responses without cell death in cultured rice cells. To elucidate the mechanism by which flg22 induced signaling in rice, we characterized OsFLS2, the rice ortholog of AtFLS2, which mediates flg22 perception. Heterologous expression of OsFLS2 functions in Arabidopsis, showing the conservation of the flg22 signaling pathway across divergent plant taxa. OsFLS2-overexpressing rice cultured cells generated stronger immune responses with the induction of cell death following stimulation with flg22 and flagellin. However, examination of the growth rate of the compatible strain in inoculated OsFLS2-overexpressing rice could not confirm bacterial growth suppression compared with wild-type rice. These results suggest that rice possesses a conserved flagellin perception system utilizing the FLS2 receptor which, when upregulated, hardly affects resistance against compatible A. avenae. |
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Authors:
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Ryota Takai; Akira Isogai; Seiji Takayama; Fang-Sik Che |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular plant-microbe interactions : MPMI Volume: 21 ISSN: 0894-0282 ISO Abbreviation: Mol. Plant Microbe Interact. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-06 Completed Date: 2008-12-18 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9107902 Medline TA: Mol Plant Microbe Interact Country: United States |
Other Details:
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Languages: eng Pagination: 1635-42 Citation Subset: IM |
Affiliation:
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Graduate School of Biological Sciences, Nara Institute of Science and Technology (NAIST), 8916-5, Takayama Ikoma, Nara 630-0191, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Arabidopsis / genetics, metabolism, microbiology Arabidopsis Proteins / genetics, metabolism Cell Death* Cells, Cultured Comamonadaceae / immunology, pathogenicity Flagellin / immunology, metabolism* Gene Expression Regulation, Plant Genes, Plant Hydrogen Peroxide / metabolism Immunity, Innate Molecular Sequence Data Oryza sativa / genetics*, immunology, metabolism, microbiology Plant Diseases / genetics, immunology Plant Proteins / genetics, metabolism* Plants, Genetically Modified / genetics, immunology, metabolism, microbiology Protein Kinases / genetics, metabolism RNA, Plant / genetics Sequence Alignment Substrate Specificity Transformation, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Plant Proteins; 0/RNA, Plant; 12777-81-0/Flagellin; 7722-84-1/Hydrogen Peroxide; EC 2.7.-/Protein Kinases; EC 2.7.1.-/FLS2 protein, Arabidopsis |
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