| Analysis of the fifth cell cycle of mouse development. | |
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MedLine Citation:
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PMID: 3184048 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The 5th cell cycle of mouse development was analyzed to determine the lengths of each cell cycle phase. The DNA content of Feulgen-stained blastomere nuclei was measured at various times throughout the cell cycle by microdensitometry. To achieve precise timing of the start of the 5th cell cycle, experiments utilized isolated 16-cell blastomeres and cell pairs obtained by in-vitro division of isolated 8-cell blastomeres. The following estimates were made for a mixed population of polar and apolar 16-cell blastomeres: G1, less than or equal to 2 h; S, 8-9 h; G2 + M, 2 h. No significant difference was found in the timing of DNA synthesis between polar and apolar cells or between cell pairs and whole embryos. |
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Authors:
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J C Chisholm |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 84 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1988 Sep |
Date Detail:
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Created Date: 1988-12-22 Completed Date: 1988-12-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 29-36 Citation Subset: IM |
Affiliation:
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Department of Anatomy, University of Cambridge, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastomeres / cytology* Cell Cycle* Cell Division Cell Nucleus / analysis DNA / analysis Densitometry Mice Mice, Inbred Strains |
| Chemical | |
Reg. No./Substance:
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9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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