Document Detail

Analysis of fatty acid steryl esters in tetraploid and hexaploid wheats: identification and comparison between chromatographic methods.
MedLine Citation:
PMID:  16159174     Owner:  NLM     Status:  MEDLINE    
Fatty acid steryl esters (FASE) in whole meal of 14 genotypes of tetraploid wheats (Triticum dicocconand T. durum) and 17 genotypes of hexaploid wheats (T. spelta and T. aestivum) were analyzed using different chromatographic strategies. By both GC-FID and HPLC-ELSD, tetraploid wheats are lacking two major peaks. The amounts of FASE, calculated on the basis of the GC-FID analysis, were double in hexaploid species as compared to tetraploids (40 and 20 mg/100 g db, respectively). HPLC with ESI-MS detection enabled the identification of FASE by the characteristic fragmentations and ion-adducts of each molecule. The distribution of steryl residues was not different between the wheat species: the main class of steryl derivatives found was the beta-sitosteryl derivatives, followed by campesteryl derivatives with small amounts of stigmasteryl esters. The esterified fatty acids explain the difference between the hexaploid and tetraploid wheats. In particular, small amounts of campesteryl and beta-sitosteryl, while no trace of stigmasteryl palmitates, were found in T. durum or its hulled ancestor T. dicoccon. Steryl oleates were not detectable in T. aestivum or its hulled ancestor T. spelta, which is consistent with the filogenesis of tetraploid and hexaploid species. Both chromatographic techniques (GC and HPLC) showed that FASE are useful to discriminate between hexaploid and tetraploid wheats from both qualitative and quantitative points of view.
Maria Fiorenza Caboni; Giovanna Iafelice; Marco Pelillo; Emanuele Marconi
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  53     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-14     Completed Date:  2005-10-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7465-72     Citation Subset:  IM    
Dipartimento di Scienze degli Alimenti, Università di Bologna, Italy.
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MeSH Terms
Chromatography / methods*
Chromatography, Gas
Chromatography, High Pressure Liquid
Esters / analysis*
Fatty Acids / analysis*
Phytosterols / analysis*
Seeds / chemistry
Spectrometry, Mass, Electrospray Ionization
Triticum / chemistry*,  genetics
Reg. No./Substance:
0/Esters; 0/Fatty Acids; 0/Phytosterols

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