Document Detail


Analysis of fatty acid composition in insulin secreting cells by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry.
MedLine Citation:
PMID:  22456534     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A comprehensive two-dimensional gas chromatography (GC×GC) time-of-flight mass spectrometry method was developed for determination of fatty acids (irrespective of origin, i.e., both free fatty acids and fatty acids bound in sources such as triglycerides) in cultured mammalian cells. The method was applied to INS-1 cells, an insulin-secreting cell line commonly used as a model in diabetes studies. In the method, lipids were extracted and transformed to fatty acid methyl esters for analysis. GC×GC analysis revealed the presence of 30 identifiable fatty acids in the extract. This result doubles the number of fatty acids previously identified in these cells. The method yielded linear calibrations and an average relative standard deviation of 8.4% for replicate injections of samples and 12.4% for replicate analysis of different samples. The method was used to demonstrate changes in fatty acid content as a function of glucose concentration on the cells. These results demonstrate the utility of this method for analysis of fatty acids in mammalian cell cultures.
Authors:
Amy L Payeur; Matthew A Lorenz; Robert T Kennedy
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-07
Journal Detail:
Title:  Journal of chromatography. B, Analytical technologies in the biomedical and life sciences     Volume:  893-894     ISSN:  1873-376X     ISO Abbreviation:  J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-04-09     Completed Date:  2012-07-26     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101139554     Medline TA:  J Chromatogr B Analyt Technol Biomed Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  187-92     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Cell Line
Fatty Acids / analysis*,  chemistry,  metabolism
Gas Chromatography-Mass Spectrometry / methods*
Glucose / pharmacology
Insulin-Secreting Cells / chemistry*,  metabolism
Metabolome / drug effects
Rats
Reproducibility of Results
Grant Support
ID/Acronym/Agency:
R37 DK046960/DK/NIDDK NIH HHS; R37 DK046960-20/DK/NIDDK NIH HHS; R37-DK046960/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 50-99-7/Glucose
Comments/Corrections

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