| Analysis of the fatty acid composition of erythrocyte phospholipids by a base catalysed transesterification method--prevention of formation of dimethylacetals. | |
| | |
MedLine Citation:
|
PMID: 3349633 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A sensitive method for the analysis of the fatty acid composition of erythrocyte phospholipids with capillary column GLC is described. Under the assay conditions (base catalysed transesterification with sodium methoxide), only the fatty acids of the phospholipids are converted to their methylesters. The alkenyl ether chains of the plasmalogens are not transmethylated. This eliminates the need for thin layer chromatography for the separation of dimethylacetals and esterified fatty acids. The method is suitable for the measurement of the fatty acid patterns of erythrocyte phospholipids in small blood samples. |
| | |
Authors:
|
M D Laryea; P Cieslicki; E Diekmann; U Wendel |
Related Documents
:
|
5810083 - N-acylphosphatidylethanolamine, a phospholipid that is rapidly metabolized during the a... 9649333 - Nonesterified fatty acids induce transmembrane monovalent cation flux: host-guest inter... 15730303 - Novel approach to 5-substituted proline derivatives using a silver-catalyzed cyclizatio... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Clinica chimica acta; international journal of clinical chemistry Volume: 171 ISSN: 0009-8981 ISO Abbreviation: Clin. Chim. Acta Publication Date: 1988 Jan |
Date Detail:
|
Created Date: 1988-05-05 Completed Date: 1988-05-05 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: NETHERLANDS |
Other Details:
|
Languages: eng Pagination: 11-8 Citation Subset: IM |
Affiliation:
|
Universitäts-Kinderklinik Düsseldorf, Abteilung für allgemeine Pädiatrie, Stoffwechselerkrankungen, FRG. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetals Catalysis Chromatography, Gas Erythrocytes / analysis* Esterification Fatty Acids / blood* Humans Hydrogen-Ion Concentration Phospholipids / blood* |
| Chemical | |
Reg. No./Substance:
|
0/Acetals; 0/Fatty Acids; 0/Phospholipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Changes in the bone and liver isoenzymes of alkaline phosphatase in postmenopausal women being treat...
Next Document: A sensitive, direct colorimetric assay of serum iron using the chromogen, nitro-PAPS.