| Analysis of the expression of putatively imprinted genes in bovine peri-implantation embryos. | |
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MedLine Citation:
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PMID: 18675451 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The application of assisted reproductive technologies (ART) has been shown to induce changes in the methylation of the embryonic genome, leading to aberrant gene expression, including that of imprinted genes. Aberrant methylation and gene expression has been linked to the large offspring syndrome (LOS) in bovine embryos resulting in increased embryonic morbidity and mortality. In the bovine, limited numbers of imprinted genes have been studied and studies have primarily been restricted to pre-implantation stages. This study reports original data on the expression pattern of 8 putatively imprinted genes (Ata3, Dlk1, Gnas, Grb10, Magel2, Mest-1, Ndn and Sgce) in bovine peri-implantation embryos. Two embryonic developmental stages were examined, Day 14 and Day 21. The gene expression pattern of single embryos was recorded for in vivo, in vitro produced (IVP) and parthenogenetic embryos. The IVP embryos allow us to estimate the effect of in vitro procedures and the analysis of parthenogenetic embryos provides provisional information on maternal genomic imprinting. Among the 8 genes investigated, only Mest-1 showed differential expression in Day 21 parthenogenetic embryos compared to in vivo and IVP counterparts, indicating maternal imprinting of this gene. In addition, our expression analysis of single embryos revealed a more heterogeneous gene expression in IVP than in in vivo developed embryos, adding further to the hypothesis of transcriptional dysregulation induced by in vitro procedures, either by in vitro maturation, fertilization or culture. In conclusion, effects of genomic imprinting and of in vitro procedures for embryo production may influence the success of bovine embryo implantation. |
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Authors:
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P Y Tveden-Nyborg; N I Alexopoulos; M A Cooney; A J French; R T Tecirlioglu; M K Holland; P D Thomsen; N T D'Cruz |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Theriogenology Volume: 70 ISSN: 0093-691X ISO Abbreviation: Theriogenology Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-15 Completed Date: 2008-12-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: United States |
Other Details:
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Languages: eng Pagination: 1119-28 Citation Subset: IM |
Affiliation:
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Dep. of Basic Animal and Veterinary Sciences, Groennegaardsvej 7, DK-1870 Frederiksberg C, Denmark. ptn@life.ku.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle / embryology* Embryo Implantation / drug effects* Embryo Transfer / veterinary Fertilization in Vitro / veterinary Gene Expression Profiling Gene Expression Regulation, Developmental / physiology* Genomic Imprinting |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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