Document Detail


Analysis of expressed sequence tags from abdominal muscle cDNA library of the pacific white shrimp Litopenaeus vannamei.
MedLine Citation:
PMID:  22443828     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The pacific white shrimp, Litopenaeus vannamei, is a popular species in aquaculture. Abdominal muscle accounts for 90% of shrimp flesh. Its growth and related genes, particularly the regulatory genes, is not well known. A cDNA library of shrimp juvenile abdominal muscle was established by PCR-based SMART™ cDNA technology. Library size was 5.0 × 106 pfu (plaque-forming unit) independent clones per microgram of starting RNA with the percentage of recombinant clones >95%. Sequence analysis of 311 randomly picked positive clones revealed 197 expressed sequence tags with average insert size of 745 nucleotides, 56% (110 of 197) clones having 5'-end sequence and 44% (87 of 197) clones having 3'-end sequence. Queries of the sequences by Blast identified 37 unknown sequences, and 160 unique clones, including 67 sequences of 100% identity matches, 28 high homologies (80% to 90% sequence match, >100 bits hit score in Blastn), 65 medium homologies (>100 bits hit score in Blastp) to the known EST sequences in the database. Among the high identity-matched ESTs, 12S ribosomal RNA, actin 1, actin 2, arginine kinase and beta-actin were the most abundant transcripts with 5 to 20 times of hit. Primary hit sequences originate from shrimp, insects, lobsters, crabs and crayfish. The EST sequences were categorized as muscle structural proteins (25%), rRNA and protein synthesis (25%), followed by mitochondrial functions (22%), exoskeleton (14%), enzymes (6%) and RNA splicing (2%), suggesting abundant and diverse transcripts present in the shrimp abdominal muscle cDNA library.
Authors:
J R Cesar; B Zhao; J Yang
Related Documents :
22277258 - Mitochondrial dna control region variation in an autochthonous basque population sample...
23900538 - Clustered protocadherins.
17099228 - Probebase--an online resource for rrna-targeted oligonucleotide probes: new features 2007.
22281208 - Phylogenetic analyses suggest a hybrid origin of the figs (moraceae: ficus) that are en...
9763288 - Hgm1, a novel male-specific gene from heterodera glycines identified by differential mr...
10553298 - Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced b...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Animal : an international journal of animal bioscience     Volume:  2     ISSN:  1751-7311     ISO Abbreviation:  Animal     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2012-03-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101303270     Medline TA:  Animal     Country:  England    
Other Details:
Languages:  eng     Pagination:  1377-83     Citation Subset:  -    
Affiliation:
1Department of Human Nutrition, Food and Animal Sciences, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Developmental potential of oocytes fertilized by conventional in vitro fertilization (IVF) or intra...
Next Document:  Herbage intake and behavioural adaptation of grazing dairy cows by restricting time at pasture under...