Document Detail


Analysis of the erythroid differentiation effect of flavonoid apigenin on K562 human chronic leukemia cells.
MedLine Citation:
PMID:  25058688     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The erythroid differentiation-inducing effect of apigenin and its derivatives on human chronic myeloid leukemia K562 has been reported but the functional group in its structure responsible for the effect has not yet been elucidated. Here, we determined the moiety responsible for the erythroid differentiation induction effect of apigenin by using different flavonoids to represent the functional groups in its structure. In addition, we compared apigenin and apigetrin, a flavonoid similar in structure to apigenin except for the glycoside in its structure. Morphological changes as well as expressions of specific markers in K562 cells treated with apigenin were compared with those treated with apigetrin, flavone, 7-hydroxyflavone, chrysin, luteolin, or naringenin. The anti-proliferative and erythroid differentiation-inducing effect of apigenin and the five flavonoids were then investigated and their effects on the α, β, and γ globin genes expressions were compared using real-time PCR. Results of the comparison between apigenin and apigetrin revealed that the glycoside part of apigetrin does not have a role in the induction of cell differentiation. Based on glycophorin A expression, the potency of the other flavonoids for induction of differentiation, was: apigenin>chrysin>flavone/7-hydroxyflavone>luteolin/naringenin. Results of the analysis of the relationship between the structure and function of the flavonoids suggest that the apigenin-induced K562 cell differentiation was due to the 2-3 double bond and hydroxyl groups in its structure. This is the first study that identified the specific functional group in apigenin that impact the erythroid differentiation effect in K562 cells.
Authors:
Hiroko Isoda; Hideko Motojima; Shoko Onaga; Imen Samet; Myra Villareal; Junkyu Han
Related Documents :
24959528 - The challenge of targeting notch in hematologic malignancies.
23186708 - Neural differentiation and support of neuroregeneration of non-neural adult stem cells.
23197858 - Characterization of autologous mesenchymal stem cell-derived neural progenitors as a fe...
24804888 - The involvement of dna methylation and histone modification on the epigenetic regulatio...
6751698 - Nitrite, nitrite alternatives, and the control of clostridium botulinum in cured meats.
8043158 - Image analysis of feulgen-stained transformed nih 3t3 cells differing in p21 expression...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-21
Journal Detail:
Title:  Chemico-biological interactions     Volume:  -     ISSN:  1872-7786     ISO Abbreviation:  Chem. Biol. Interact.     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0227276     Medline TA:  Chem Biol Interact     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014. Published by Elsevier Ireland Ltd.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Inhibition of Campylobacter jejuni on fresh chicken breasts by ?-carrageenan/chitosan-based coatings...
Next Document:  Preterm birth in the first pregnancy and risk of neonatal death in the second pregnancy: A propensit...