Document Detail


Analysis of eosinophilic round bodies formed after injection of enamel matrix derivative into the backs of rats.
MedLine Citation:
PMID:  16274313     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Enamel matrix derivative (EMD) is used in dental clinics for the regeneration of alveolar bone. Its effects have not yet been clarified, although it induces eosinophilic round bodies (ERBs) and cartilage formation at the injection site. The objective of this experiment was to examine the histopathologic and biochemical properties of ERBs formed after EMD injection. METHODS: The backs of Sprague-Dawley rats injected with various concentrations of EMD were examined histopathologically. For biochemical examinations, ERBs were microdissected out from the sections. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), matrix-assisted laser-desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS), and database analysis of ERBs were carried out. RESULTS: The histopathological findings were consistent with a foreign body reaction. Numerous ERBs were observed 7 days after injection of 30.0 mg/ml EMD. Histopathologically, ERBs did not contain polysaccharide, amyloid, or hemosiderin. The cells surrounding ERBs were not macrophages or vascular endothelial cells. SDS-PAGE of the microdissected ERBs revealed an intense band at around the 40-kDa region. MALDI-TOF MS showed that the spectrum for ERBs has only a single strong ion intensity. Analysis of the amino acid sequence revealed that the ERBs were composed of various molecular fragments, which all contained an identical seven amino acid sequence. In addition, these peptides are a component of amelogenin. CONCLUSIONS: A high concentration of EMD induces ERBs that consist of a 40-kDa protein which includes a constituent part of amelogenin. The ERBs (or remaining EMD) might promote mesenchymal cell differentiation into hard tissue-forming cells around the EMD injection site.
Authors:
Nak-Hyun Kim; Kazuya Tominaga; Akio Tanaka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of periodontology     Volume:  76     ISSN:  0022-3492     ISO Abbreviation:  J. Periodontol.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-08     Completed Date:  2006-02-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1934-41     Citation Subset:  D; IM    
Affiliation:
Department of Oral Pathology, Osaka Dental University, Osaka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amelogenin
Animals
Chondrogenesis / drug effects
Connective Tissue / drug effects,  pathology
Cytoplasmic Structures / drug effects,  ultrastructure
Dental Enamel Proteins / administration & dosage,  analysis,  pharmacology*
Electrophoresis, Polyacrylamide Gel
Foreign-Body Reaction / chemically induced,  pathology
Injections, Subcutaneous
Male
Mass Spectrometry
Microdissection
Peptide Fragments / analysis
Rats
Rats, Sprague-Dawley
Sequence Analysis, Protein
Skin / drug effects*,  pathology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical
Reg. No./Substance:
0/Amelogenin; 0/Amelx protein, rat; 0/Dental Enamel Proteins; 0/Peptide Fragments; 0/enamel matrix proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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