Document Detail


Analysis of the envelope (E) protein gene of tick-borne encephalitis viruses isolated in South Korea.
MedLine Citation:
PMID:  19480604     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We determined the nucleotide and deduced amino acid sequences of the complete envelope (E) protein gene of the five tick-borne encephalitis virus (TBEV) strains KrM 93, KrM 213, KrM 215, KrM 216, and KrM 219, isolated from wild rodents in South Korea. We analyzed genetic variability within the isolates and compared them with 13 other TBEV strains. The complete E protein genes were amplified by reverse transcription polymerase chain reaction (RT-PCR), cloned into pGEM-T vectors, and sequenced. The five isolates were similar to the Western subtype in nucleotide and deduced amino acid sequences (97%-99% identity) and by phylogenetic analysis. The deduced amino acid alignments had 13 unique amino acids, as in the Western subtypes. Among the signature amino acids, those at positions 206 and 317 were unique to each subtype. We were also able to identify amino acid substitutions in each of the three domains when comparing the 5 Korean isolates with the 13 other TBEV strains. Thus, we confirmed that the 5 Korean isolates belong to the Western subtype. These data will provide useful information for the development of an effective recombinant vaccine.
Authors:
Seok-Min Yun; Su-Yeon Kim; Myung Guk Han; Young Eui Jeong; Tai-Soon Yong; Chan-Hee Lee; Young Ran Ju
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Vector borne and zoonotic diseases (Larchmont, N.Y.)     Volume:  9     ISSN:  1557-7759     ISO Abbreviation:  Vector Borne Zoonotic Dis.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-18     Completed Date:  2009-09-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100965525     Medline TA:  Vector Borne Zoonotic Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  287-93     Citation Subset:  IM    
Affiliation:
Division of Arboviruses, Center for Immunology & Pathology, National Institute of Health, Centers for Disease Control and Prevention, Seoul, Korea.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Cell Line
Cricetinae
Encephalitis Viruses, Tick-Borne / genetics*,  physiology*
Gene Expression Regulation, Viral / physiology
Korea
Mice
Molecular Sequence Data
Phylogeny
Viral Envelope Proteins / chemistry,  genetics*,  metabolism*
Chemical
Reg. No./Substance:
0/Viral Envelope Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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