Document Detail


Analysis of the effect of industrial effluent on pigments, proteins, nucleic acids, and the 2,6-dichlorophenol indophenol Hill reaction of rice seedlings.
MedLine Citation:
PMID:  6192989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of an industrial effluent on cellular macromolecular composition and indophenol photochemical activity of isolated chloroplasts of rice (Orvza sativa L. C. V. Mushoori) seedlings have been investigated. Each experiment consisted of two parts. One was the effect of various concentrations and the other was the time-dependent changes induced by the undiluted effluent. The total pigments, proteins, and nucleic acids of rice seedlings declined with an increase in effluent concentration and the time of incubation. The loss in contents of macromolecules like deoxyribonucleic acid (DNA), ribonucleic acid (RNA), and protein was relatively more marked in the root than in the shoot. RNA and chlorophyll (Chl) contents of the seedlings were found most susceptible to effluent stress. Loss in Hill reaction activity measured as photoreduction of 2,6-dichlorophenol indophenol (DCPIP) of isolated chloroplasts could be correlated in a general way with the loss of pigments, proteins and nucleic acids. Diphenyl carbazide- and Mn2+-induced restoration of loss in DCPIP photoreduction suggests that the damage of oxygen evolving systems is the initial site of action of the effluent.
Authors:
B K Behera; B N Misra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental research     Volume:  31     ISSN:  0013-9351     ISO Abbreviation:  Environ. Res.     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1983-10-08     Completed Date:  1983-10-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0147621     Medline TA:  Environ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
2,6-Dichloroindophenol
DNA / metabolism*
Industrial Waste / adverse effects*
Macromolecular Substances
Oryza sativa / drug effects*,  metabolism
Photochemistry
Photosynthesis / drug effects*
Pigments, Biological / metabolism*
Plant Proteins / metabolism*
RNA / metabolism*
Chemical
Reg. No./Substance:
0/Industrial Waste; 0/Macromolecular Substances; 0/Pigments, Biological; 0/Plant Proteins; 63231-63-0/RNA; 9007-49-2/DNA; 956-48-9/2,6-Dichloroindophenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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