| Analysis of differentially expressed genes in response to bacterial stimulation in hemocytes of the carpet-shell clam Ruditapes decussatus: identification of new antimicrobial peptides. | |
| | |
MedLine Citation:
|
PMID: 17881157 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Suppression subtractive hybridization was used to identify differentially expressed genes in hemocytes from carpet-shell clam Ruditapes decussatus stimulated with a mixture of dead bacterial strains. Putative function could be assigned to 100 of the 253 sequenced cDNAs. Based on sequence homologies, 3.16% of the total identified genes were possibly related to immune functions. Clam myticin isoforms 1, 2 and 3, and clam mytilin, with similarity with myticins and mytilins previously reported on Mytilus galloprovincialis were identified and characterized for the first time in clams. The analysis of their expression levels by quantitative PCR showed that they were induced by bacterial challenge. The results obtained in this work could be the first step leading to the understanding of molecular mechanisms by which these economically important marine bivalves respond to pathogens. |
| | |
Authors:
|
Camino Gestal; Marímar Costa; Antonio Figueras; Beatriz Novoa |
Related Documents
:
|
9889007 - Characterization of a novel gene, pnutl2, on human chromosome 17q22-q23 and its exclusi... 17855167 - The evolution of immunity: a low-life perspective. 10964057 - Gene expression profiles in squamous cell carcinomas of the oral cavity: use of laser c... 11167527 - Mapping of 14 expressed sequence tags (ests) from porcine skeletal muscle by somatic ce... 9282787 - Generation of an infectious cdna of a highly cardiovirulent coxsackievirus b3(cvb3m) an... 16952937 - The hmuq and hmud genes from bradyrhizobium japonicum encode heme-degrading enzymes. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-08-15 |
Journal Detail:
|
Title: Gene Volume: 406 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 2007 Dec |
Date Detail:
|
Created Date: 2007-12-11 Completed Date: 2008-02-28 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 7706761 Medline TA: Gene Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 134-43 Citation Subset: IM |
Affiliation:
|
Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Eduardo Cabello 6, Vigo, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Animals Antimicrobial Cationic Peptides / genetics* Base Sequence Bivalvia / genetics* Blood Proteins / genetics* Expressed Sequence Tags Gene Expression Hemocytes / metabolism* Immunity, Innate / genetics* Molecular Sequence Data Phylogeny |
| Chemical | |
Reg. No./Substance:
|
0/Antimicrobial Cationic Peptides; 0/Blood Proteins; 0/myticin; 0/mytilin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transcriptional regulation of a new variant of human platelet-derived growth factor receptor alpha t...
Next Document: Genomic analyses of bovine viral diarrhea viruses isolated from cattle imported into Japan between 1...