Document Detail


[Analysis of differentially expressed genes from female and male postparasitic Ovomermis sinensis juveniles].
MedLine Citation:
PMID:  23019030     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Mermithidae, as an important natural predator of pests such as Bollworm, has great potential for natural biological control of invasive pests. Unfortunately, the in vitro culture of the nematode has not yet been successful, delaying the commercial application of this pest control method. The key reason for this failure is the inability of the worms to accomplish sex differentiation, sparking a strong interest in this process. Here, we analyzed the differences in gene expression of female and male postparasitic Ovomermis sinensis juveniles by mRNA differential display. In total, 20 gene fragments that had differential expression in male and female worms were isolated, including 8 male- and 12 female-specific ones. Bioinformatics methods were employed to analyze sequences of these fragments, in which ensembl analysis shows 4 fragments have comparable parts with C. elegence's X chromosome, we speculate those fragments are important genes which influence sex differentiation of Ovomermis sinensis, This data provides an idea for further study of the molecular mechanism of sex differentiation in mermithids.
Authors:
Yun-Mei Wu; Li-Hong Zhang; Guo-Xiu Wang
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Dong wu xue yan jiu = Zoological research / "Dong wu xue yan jiu" bian ji wei yuan hui bian ji     Volume:  33     ISSN:  0254-5853     ISO Abbreviation:  Zool. Res.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100961499     Medline TA:  Dongwuxue Yanjiu     Country:  China    
Other Details:
Languages:  chi     Pagination:  487-92     Citation Subset:  IM    
Affiliation:
Hubei Key Laboratory of Genetic and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, China. wangguoxiu@ yahoo.com.cn.
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