| Analysis of the difference in color development in the dye-binding method due to the kind of buffer solution. | |
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MedLine Citation:
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PMID: 16772695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a dye-binding method using a pH indicator, color development has reportedly been affected by the kind of buffer solution used in the color reagent. This phenomenon was analyzed by using a calculation based on the assumption that the anion of the buffer solution also reacts with protein. Color development decreases with increases in the anion concentration of the buffer solution and in the equilibrium constant of the reaction between the anion and protein. The differences in color development due to the kind of buffer solution can be attributed to differences in the equilibrium constant of the reaction forming the anion-protein complex and to the concentration of the anion between the buffer solutions. |
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Authors:
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Yuji Suzuki |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry Volume: 22 ISSN: 0910-6340 ISO Abbreviation: Anal Sci Publication Date: 2006 Jun |
Date Detail:
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Created Date: 2006-06-14 Completed Date: 2007-05-24 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8511078 Medline TA: Anal Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 907-10 Citation Subset: IM |
Affiliation:
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Department of Medical Technology, Saitama Prefectural University Junior College, Koshigaya, Saitama, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anions Buffers* Chemistry Techniques, Analytical / methods* Citric Acid / chemistry Colorimetry / methods* Coloring Agents / analysis*, chemistry, pharmacology Hydrogen / chemistry Hydrogen-Ion Concentration Indicators and Reagents / analysis*, chemistry Ions Kinetics Models, Chemical Phosphates / chemistry Proteins / analysis |
| Chemical | |
Reg. No./Substance:
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0/Anions; 0/Buffers; 0/Coloring Agents; 0/Indicators and Reagents; 0/Ions; 0/Phosphates; 0/Proteins; 1333-74-0/Hydrogen; 77-92-9/Citric Acid |
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