Document Detail


Analysis of the difference in color development in the dye-binding method due to the kind of buffer solution.
MedLine Citation:
PMID:  16772695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a dye-binding method using a pH indicator, color development has reportedly been affected by the kind of buffer solution used in the color reagent. This phenomenon was analyzed by using a calculation based on the assumption that the anion of the buffer solution also reacts with protein. Color development decreases with increases in the anion concentration of the buffer solution and in the equilibrium constant of the reaction between the anion and protein. The differences in color development due to the kind of buffer solution can be attributed to differences in the equilibrium constant of the reaction forming the anion-protein complex and to the concentration of the anion between the buffer solutions.
Authors:
Yuji Suzuki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  22     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-06-14     Completed Date:  2007-05-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  907-10     Citation Subset:  IM    
Affiliation:
Department of Medical Technology, Saitama Prefectural University Junior College, Koshigaya, Saitama, Japan.
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MeSH Terms
Descriptor/Qualifier:
Anions
Buffers*
Chemistry Techniques, Analytical / methods*
Citric Acid / chemistry
Colorimetry / methods*
Coloring Agents / analysis*,  chemistry,  pharmacology
Hydrogen / chemistry
Hydrogen-Ion Concentration
Indicators and Reagents / analysis*,  chemistry
Ions
Kinetics
Models, Chemical
Phosphates / chemistry
Proteins / analysis
Chemical
Reg. No./Substance:
0/Anions; 0/Buffers; 0/Coloring Agents; 0/Indicators and Reagents; 0/Ions; 0/Phosphates; 0/Proteins; 1333-74-0/Hydrogen; 77-92-9/Citric Acid

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