Document Detail

Analysis of the desialidation process of the haemagglutinin protein of influenza B virus: the host-dependent desialidation step.
MedLine Citation:
PMID:  12075092     Owner:  NLM     Status:  MEDLINE    
It was reported previously that haemadsorption by the haemagglutinin (HA) protein of influenza B virus required that the protein must undergo desialidation. When MDCK and COS cells were infected with influenza B/Kanagawa/73 virus in the presence of a neuraminidase (NA) inhibitor, Zanamivir, haemadsorption on MDCK cells was inhibited but that on COS cells was not. The activity of the NA protein of the two types of infected cells was similar and both were inhibited by Zanamivir in a dose-dependent manner. A comparison of the desialidation of the HA protein was made on MDCK and COS cells in the presence of bacterial NA and both cells were found to have similar sensitivity. On the accumulation of the HA and NA proteins in the trans-Golgi network of MDCK cells by means of low-temperature treatment, desialidation of the HA protein in the presence of Zanamivir was detected by two-dimensional gel electrophoresis. Because this agent was reported to be unable to penetrate cells, these data suggest that, in MDCK cells, desialidation of the HA protein occurs on the cell surface but, in COS cells, the HA and NA proteins might accumulate in the trans-Golgi network, thus allowing NA desialidation before their migration to the cell surface.
C Luo; E Nobusawa; K Nakajima
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of general virology     Volume:  83     ISSN:  0022-1317     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-20     Completed Date:  2002-09-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1729-34     Citation Subset:  IM    
Department of Virology, Medical School, Nagoya City University, 1 Kawasumi, Mizuho-chou, Mizuho-ku, Nagoya 467-8601, Japan.
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MeSH Terms
COS Cells
Cell Line
Cercopithecus aethiops
Dose-Response Relationship, Drug
Electrophoresis, Gel, Two-Dimensional
Golgi Apparatus / metabolism
Hemadsorption / drug effects
Hemagglutinin Glycoproteins, Influenza Virus / chemistry,  metabolism*
Influenza B virus / immunology,  physiology*
N-Acetylneuraminic Acid / metabolism
Neuraminidase / antagonists & inhibitors,  pharmacology
Sialic Acids / pharmacology
Species Specificity
Virus Replication
Reg. No./Substance:
0/Guanidines; 0/Hemagglutinin Glycoproteins, Influenza Virus; 0/Pyrans; 0/Sialic Acids; 131-48-6/N-Acetylneuraminic Acid; 139110-80-8/Zanamivir; EC

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