| Analysis of cytokine secretion from lymphocytes of patients with hypersensitivity reactions to contaminated heparins. | |
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MedLine Citation:
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PMID: 20854434 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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BACKGROUND: Beginning in 2007, anaphylactoid reactions associated with unfractionated heparin (UFH) occurred and resulted in some fatalities. These reactions were reported to be linked to the complement and contact system activation induced by certain batches of UFH containing the adulterant oversulphated chondroitin sulphate (OSCS). OBJECTIVES: Drug-specific secretion of selected cytokines from peripheral blood mononuclear cells (PBMC) of patients with hypersensitivity reactions to contaminated heparin was compared with the respective in vitro cytokine pattern of individuals with or without hypersensitivity to heparin, different glycosaminoglycans or other drugs. METHODS: Study individuals (n = 13) were classified as follows: patients with hypersensitivity reactions to contaminated (OSCS) heparin (n = 3), noncontaminated heparin (n = 1) or other compounds (n = 3) and patients with ongoing heparin therapy without symptoms of intolerance (n = 2). Four healthy individuals served as controls. PBMC were incubated with six different glycosaminoglycan structures. Drug-specific intracellular interleukin (IL)-5, interferon (IFN)-γ and IL-10 production was investigated by flow cytometry, while secretion of IL-5, IL-2 and IFN-γ was analysed by enzyme-linked immunosorbent assay. RESULTS: PBMC from individuals with hypersensitivity reactions to contaminated heparin secreted considerable amounts of IL-2 in vitro. There was a suggestion that ongoing heparin therapy and the Li-heparin in the vials may have an impact on the lymphocyte reactivity of PBMC. CONCLUSIONS: The in vitro lymphocyte reactivity pattern of PBMC from individuals with hypersensitivity reactions to contaminated heparins was neither typical for an immune-mediated nor for a nonimmune-mediated reaction. Possible effects of heparins in the test system itself may require consideration. |
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Authors:
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M Martin; H Ott; H F Merk; B Sachs |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The British journal of dermatology Volume: 164 ISSN: 1365-2133 ISO Abbreviation: Br. J. Dermatol. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2010-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0004041 Medline TA: Br J Dermatol Country: England |
Other Details:
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Languages: eng Pagination: 68-75 Citation Subset: IM |
Copyright Information:
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© 2011 The Authors. BJD © 2011 British Association of Dermatologists 2011. |
Affiliation:
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Federal Institute for Drugs and Medical Devices, 53175 Bonn, Germany. mail@marion-martin.info |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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