Document Detail

Analysis of conjugated linoleic acids as 9-anthrylmethyl esters by reversed-phase high-performance liquid chromatography with fluorescence detection.
MedLine Citation:
PMID:  16212797     Owner:  NLM     Status:  MEDLINE    
A simple and highly sensitive method for determining the fatty acid composition of food lipids containing conjugated linoleic acid (CLA) is described. The method is based on the separation of the 9-anthrylmethyl ester derivatives of saturated and unsaturated (conjugated and non-conjugated) fatty acids by reversed-phase high-performance liquid chromatography with fluorescence detection. Just like the other fatty acids, CLA reacts readily with 9-anthryldiazomethane at room temperature to produce 9-anthrylmethyl esters without isomerization and decomposition of the conjugated double bonds. Clear resolution of the individual fatty acids as their 9-anthrylmethyl esters is achieved on a highly efficient octadecylsilylated silica column (150- x 3-mm i.d., 3-microm particle size) using a stepwise gradient elution with methanol-water. The method is standardized with commercially available CLA isomers (cis-9, trans-11 and trans-10, cis-12-octadecadienoic acids, and their cis,cis and trans,trans isomers) and applied for determination of the fatty acid compositions of milk and sdairy products.
Kazuhiko Nishimura; Toshiyuki Suzuki; Svetlana Momchilova; Kazuo Miyashita; Eiji Katsura; Yutaka Itabashi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  43     ISSN:  0021-9665     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-10-10     Completed Date:  2006-01-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  494-9     Citation Subset:  IM    
Graduate School of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan.
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MeSH Terms
Chromatography, High Pressure Liquid / methods*
Dairy Products / analysis
Linoleic Acids, Conjugated / analysis*,  chemistry
Spectrometry, Fluorescence / methods*
Reg. No./Substance:
0/Esters; 0/Linoleic Acids, Conjugated

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