| Analysis of conjugated linoleic acids as 9-anthrylmethyl esters by reversed-phase high-performance liquid chromatography with fluorescence detection. | |
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MedLine Citation:
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PMID: 16212797 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and highly sensitive method for determining the fatty acid composition of food lipids containing conjugated linoleic acid (CLA) is described. The method is based on the separation of the 9-anthrylmethyl ester derivatives of saturated and unsaturated (conjugated and non-conjugated) fatty acids by reversed-phase high-performance liquid chromatography with fluorescence detection. Just like the other fatty acids, CLA reacts readily with 9-anthryldiazomethane at room temperature to produce 9-anthrylmethyl esters without isomerization and decomposition of the conjugated double bonds. Clear resolution of the individual fatty acids as their 9-anthrylmethyl esters is achieved on a highly efficient octadecylsilylated silica column (150- x 3-mm i.d., 3-microm particle size) using a stepwise gradient elution with methanol-water. The method is standardized with commercially available CLA isomers (cis-9, trans-11 and trans-10, cis-12-octadecadienoic acids, and their cis,cis and trans,trans isomers) and applied for determination of the fatty acid compositions of milk and sdairy products. |
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Authors:
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Kazuhiko Nishimura; Toshiyuki Suzuki; Svetlana Momchilova; Kazuo Miyashita; Eiji Katsura; Yutaka Itabashi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatographic science Volume: 43 ISSN: 0021-9665 ISO Abbreviation: J Chromatogr Sci Publication Date: 2005 Oct |
Date Detail:
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Created Date: 2005-10-10 Completed Date: 2006-01-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0173225 Medline TA: J Chromatogr Sci Country: United States |
Other Details:
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Languages: eng Pagination: 494-9 Citation Subset: IM |
Affiliation:
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Graduate School of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Chromatography, High Pressure Liquid / methods* Dairy Products / analysis Esters Linoleic Acids, Conjugated / analysis*, chemistry Spectrometry, Fluorescence / methods* |
| Chemical | |
Reg. No./Substance:
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0/Esters; 0/Linoleic Acids, Conjugated |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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