Document Detail


Analysis of composition of sugar meals of wild mosquitoes by gas chromatography.
MedLine Citation:
PMID:  10084129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gas chromatography (GC) was successfully used for the first time to determine the components of natural sugar meals in individual mosquitoes and to determine whether carbohydrases are present in the crops of these insects. Crops of wild mosquitoes collected from a 2-ha cypress swamp north of Gainesville, FL, contained fructose, glucose, sucrose, maltose, turanose, melibiose, erlose, melezitose, raffinose, and a few unidentified carbohydrates. Time course studies with male and female Aedes albopictus showed rapid hydrolysis (> 90%) of sucrose occurring within 2 h of ingestion, whereas melezitose remained relatively unchanged even 8 h after ingestion. The crop extraction/GC analysis technique is an improvement over the cold anthrone test traditionally used for sugar analysis. This procedure is a rapid one-step process used to determine natural sugar sources, hydrolysis, occurrence, and preferences for individual wild sugar-feeding Diptera.
Authors:
D A Burkett; D A Carlson; D L Kline
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of the American Mosquito Control Association     Volume:  14     ISSN:  8756-971X     ISO Abbreviation:  J. Am. Mosq. Control Assoc.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-05-10     Completed Date:  1999-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8511299     Medline TA:  J Am Mosq Control Assoc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  373-9     Citation Subset:  IM    
Affiliation:
Department of Entomology and Nematology, University of Florida, Gainesville 32611-0620, USA.
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MeSH Terms
Descriptor/Qualifier:
Aedes*
Animals
Chromatography, Gas
Dietary Sucrose / analysis*,  metabolism
Female
Glycoside Hydrolases / metabolism
Hydrolysis
Male
Chemical
Reg. No./Substance:
0/Dietary Sucrose; EC 3.2.1.-/Glycoside Hydrolases; EC 3.2.1.-/carbohydrase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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