| Analysis and comparison of the bacterial community in fermented grains during the fermentation for two different styles of Chinese liquor. | |
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MedLine Citation:
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PMID: 18317829 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Bacterial populations in fermented grains during fermentation may play important roles in Chinese liquor flavor. PCR-based denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene library analysis were performed to analyze the bacterial community structure of two styles of liquor. The results of DGGE profiles showed that bacterial diversity decreased with the fermentation process and Lactobacillus acetotolerans became the predominant species at the end of the fermentation. But the obvious differences of bacterial community appeared in the middle stage of two styles of liquor fermentation, in which the different upstream production techniques were used. Moreover, 16S rRNA gene libraries of two styles were constructed. A total of 125 and 107 clones, chosen from two libraries, were grouped into 46 and 49 operational taxonomic units (OTUs) by amplified ribosomal DNA restriction analysis. According to sequencing results of clones, the predominant bacteria in strong aroma style fermented grains were those from the class Bacilli, Bacteroidetes, and Clostridia, whereas the predominant bacteria in fermented grains of roasted sesame aroma style belonged to Bacilli, Flavobacteria, and Gammaproteobacteria. Molecular analysis of the bacterial diversity of the liquor fermentation will benefit the analysis of important microorganisms playing key roles in the formation of liquor flavor components. |
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Authors:
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Hai-Yan Wang; Xiao-Jun Zhang; Li-Ping Zhao; Yan Xu |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-04 |
Journal Detail:
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Title: Journal of industrial microbiology & biotechnology Volume: 35 ISSN: 1367-5435 ISO Abbreviation: J. Ind. Microbiol. Biotechnol. Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-14 Completed Date: 2008-08-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9705544 Medline TA: J Ind Microbiol Biotechnol Country: England |
Other Details:
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Languages: eng Pagination: 603-9 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Food Science and Technology, Key Laboratory of Industrial Biotechnology of Ministry of Education and School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, 214122 Wuxi, Jiangsu, China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/EU195444; EU195445; EU195446; EU195447; EU195448 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
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classification,
genetics,
growth & development*,
isolation & purification* Biodiversity Cereals / microbiology* China Electrophoresis, Polyacrylamide Gel Fermentation* Food Microbiology* Lactobacillus / genetics, isolation & purification Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S / genetics |
| Chemical | |
Reg. No./Substance:
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0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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