| Analysis of circulating microRNA biomarkers in plasma and serum using quantitative reverse transcription-PCR (qRT-PCR). | |
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MedLine Citation:
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PMID: 20146939 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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MicroRNAs (miRNAs) are small (approximately 22 nt) RNAs that play important roles in gene regulatory networks by binding to and repressing the activity of specific target mRNAs. Recent studies have indicated that miRNAs circulate in a stable, cell-free form in the bloodstream and that the abundance of specific miRNAs in plasma or serum can serve as biomarkers of cancer and other diseases. Measurement of circulating miRNAs as biomarkers is associated with some special challenges, including those related to pre-analytic variation and data normalization. We describe here our procedure for qRT-PCR analysis of circulating miRNAs as biomarkers, and discuss relevant issues of sample preparation, experimental design and data analysis. |
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Authors:
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Evan M Kroh; Rachael K Parkin; Patrick S Mitchell; Muneesh Tewari |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-02-08 |
Journal Detail:
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Title: Methods (San Diego, Calif.) Volume: 50 ISSN: 1095-9130 ISO Abbreviation: Methods Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-10 Completed Date: 2010-05-28 Revised Date: 2010-11-16 |
Medline Journal Info:
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Nlm Unique ID: 9426302 Medline TA: Methods Country: United States |
Other Details:
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Languages: eng Pagination: 298-301 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Human Biology Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Markers
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blood* Humans MicroRNAs / blood* Nucleic Acid Amplification Techniques Plasma / chemistry Quality Control Reverse Transcriptase Polymerase Chain Reaction / methods* Serum / chemistry Tumor Markers, Biological / blood |
| Grant Support | |
ID/Acronym/Agency:
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5 P30 CA015704/CA/NCI NIH HHS; P50 CA83636/CA/NCI NIH HHS; P50 CA97186/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/MicroRNAs; 0/Tumor Markers, Biological |
| Comments/Corrections | |
Erratum In:
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Methods. 2010 Nov;52(3):268 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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