| Analysis of ciprofloxacin by a simple high-performance liquid chromatography method. | |
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MedLine Citation:
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PMID: 18647468 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and sensitive high-performance liquid chromatographic method is described for the quantitative analysis of ciprofloxacin in pharmaceuticals and human plasma. The method employs reversed-phase chromatography using an RP-C18 column with an isocratic mobile phase of acetonitrile-2% acetic acid aqueous solution (16:84, v/v), umbelliferone as an internal standard, and a flow rate of 1.0 mL/min. The UV detector is set at 280 nm. The limit of detection is 0.25 microM (S/N = 3, injection volume = 10 microL). The regression equations are linear (r > 0.9999) over a range between 0.51 approximately 130 microM for the pharmaceutical analysis of ciprofloxacin and 0.51 approximately 64.8 microM for the biological analysis of ciprofloxacin in human plasma. The intra- and inter-day relative standard deviation and relative error are less than 3.39% and 5.71%, respectively. All the recoveries are greater than 93.8%. This method is successfully applied in a pharmacokinetic study of a volunteer who receives a 500 mg ciprofloxacin tablet. |
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Authors:
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Shihn-Sheng Wu; Chih-Yuan Chein; Yen-Hsia Wen |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chromatographic science Volume: 46 ISSN: 0021-9665 ISO Abbreviation: J Chromatogr Sci Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-07-23 Completed Date: 2008-08-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0173225 Medline TA: J Chromatogr Sci Country: United States |
Other Details:
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Languages: eng Pagination: 490-5 Citation Subset: IM |
Affiliation:
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Pharmacy School, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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analysis*,
blood,
pharmacokinetics Ciprofloxacin / analysis*, blood, pharmacokinetics Humans Pharmaceutical Preparations / chemistry* Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Pharmaceutical Preparations; 85721-33-1/Ciprofloxacin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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