Document Detail

Analysis of ciprofloxacin by a simple high-performance liquid chromatography method.
MedLine Citation:
PMID:  18647468     Owner:  NLM     Status:  MEDLINE    
A simple and sensitive high-performance liquid chromatographic method is described for the quantitative analysis of ciprofloxacin in pharmaceuticals and human plasma. The method employs reversed-phase chromatography using an RP-C18 column with an isocratic mobile phase of acetonitrile-2% acetic acid aqueous solution (16:84, v/v), umbelliferone as an internal standard, and a flow rate of 1.0 mL/min. The UV detector is set at 280 nm. The limit of detection is 0.25 microM (S/N = 3, injection volume = 10 microL). The regression equations are linear (r > 0.9999) over a range between 0.51 approximately 130 microM for the pharmaceutical analysis of ciprofloxacin and 0.51 approximately 64.8 microM for the biological analysis of ciprofloxacin in human plasma. The intra- and inter-day relative standard deviation and relative error are less than 3.39% and 5.71%, respectively. All the recoveries are greater than 93.8%. This method is successfully applied in a pharmacokinetic study of a volunteer who receives a 500 mg ciprofloxacin tablet.
Shihn-Sheng Wu; Chih-Yuan Chein; Yen-Hsia Wen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of chromatographic science     Volume:  46     ISSN:  0021-9665     ISO Abbreviation:  J Chromatogr Sci     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-23     Completed Date:  2008-08-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0173225     Medline TA:  J Chromatogr Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  490-5     Citation Subset:  IM    
Pharmacy School, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan.
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MeSH Terms
Anti-Bacterial Agents / analysis*,  blood,  pharmacokinetics
Ciprofloxacin / analysis*,  blood,  pharmacokinetics
Pharmaceutical Preparations / chemistry*
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Pharmaceutical Preparations; 85721-33-1/Ciprofloxacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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