Document Detail

Analysis of chromosomal abnormalities in human sperm after chemotherapy by karyotyping and fluorescence in situ hybridization (FISH).
MedLine Citation:
PMID:  7535187     Owner:  NLM     Status:  MEDLINE    
The frequency of numerical and structural chromosomal abnormalities was studied in the sperm of a lymphoma patient 3 years after MACOP-B chemotherapy (CT). Sperm karyotyping was performed by fusion of human sperm with hamster oocytes and analysis of 193 Q-banded sperm chromosomes. Multicolor fluorescence in situ hybridization (FISH) was performed on 10,228 sperm for analysis of disomy frequencies for chromosomes 1 and 12 and on 10,664 sperm for chromosomes X and Y. Sperm karyotyping demonstrated numerical abnormalities in 7.3% of the spreads, 6.7% hypohaploid and 0.5% hyperhaploid, giving a conservative estimate of aneuploidy of 1%. Structural chromosomal abnormalities were present in 7.3% of the karyotypes and 0.5% had both numerical and structural abnormalities. Results of FISH analyses yielded disomy frequencies of 0.10%, 0.11%, 0.04%, 0.05%, and 0.18% for chromosomes 1, 12, X, Y, and XY, respectively. The frequency of diploid sperm was 0.09%. The frequency of abnormalities was not significantly increased compared to control donors for any of the studies. Also, the frequencies of X- and Y-bearing sperm did not differ significantly from 50% in the sperm karyotyping or FISH studies.
R H Martin; A W Rademaker; N J Leonard
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  80     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-04-28     Completed Date:  1995-04-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  29-32     Citation Subset:  IM    
Department of Medical Genetics, Faculty of Medicine, University of Calgary, Alberta, Canada.
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MeSH Terms
Antineoplastic Combined Chemotherapy Protocols / therapeutic use*
Bleomycin / therapeutic use
Chromosome Aberrations*
Cyclophosphamide / therapeutic use
Doxorubicin / therapeutic use
In Situ Hybridization, Fluorescence
Leucovorin / therapeutic use
Lymphoma / drug therapy*
Methotrexate / therapeutic use
Prednisone / therapeutic use
Spermatozoa / ultrastructure*
Vincristine / therapeutic use
Reg. No./Substance:
0/MACOP-B protocol; 11056-06-7/Bleomycin; 23214-92-8/Doxorubicin; 50-18-0/Cyclophosphamide; 53-03-2/Prednisone; 57-22-7/Vincristine; 58-05-9/Leucovorin; 59-05-2/Methotrexate

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