Document Detail


Analysis of a challenging subset of World Anti-Doping Agency-banned steroids and antiestrogens by LC-MS-MS.
MedLine Citation:
PMID:  17579958     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A qualitative liquid chromatography-tandem mass spectrometry method for the analysis of 22 sporting federation-banned anabolic agents (or their metabolite markers) and anti-estrogens in urine that are refractory to analysis by gas chromatography-mass spectrometry is presented. In addition, a quantitative method built around World Anti-Doping Agency (WADA) guidelines for the confirmatory analysis of 19-norandrosterone, the primary metabolite of nandrolone with a WADA-specified minimum required performance limit of 1 ng/mL, is included. Hydrolysis of glucuronide conjugates, liquid-liquid extraction, no clean-up derivatization with Girard's Reagent P, and analysis by quadrupole-time-of-flight mass spectrometry provide sensitivity and selectivity well beyond that required by the WADA.
Authors:
Chad R Borges; Nicole Miller; Melinda Shelby; Megan Hansen; Claudia White; Matthew H Slawson; Kim Monti; Dennis J Crouch
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Publication Detail:
Type:  Journal Article; Validation Studies    
Journal Detail:
Title:  Journal of analytical toxicology     Volume:  31     ISSN:  0146-4760     ISO Abbreviation:  J Anal Toxicol     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-06-20     Completed Date:  2007-07-11     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7705085     Medline TA:  J Anal Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  125-31     Citation Subset:  IM    
Affiliation:
Sports Medicine Research and Testing Laboratory, Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, Utah 84108, USA.
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MeSH Terms
Descriptor/Qualifier:
Anabolic Agents / urine*
Chromatography, High Pressure Liquid*
Doping in Sports*
Estranes / urine*
Estrogen Receptor Modulators / urine*
Guidelines as Topic
Humans
International Agencies
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization*
Substance Abuse Detection / methods*
Tandem Mass Spectrometry*
Chemical
Reg. No./Substance:
0/Anabolic Agents; 0/Estranes; 0/Estrogen Receptor Modulators; 65556-19-6/19-norandrosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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