| Analysis of a challenging subset of World Anti-Doping Agency-banned steroids and antiestrogens by LC-MS-MS. | |
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MedLine Citation:
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PMID: 17579958 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A qualitative liquid chromatography-tandem mass spectrometry method for the analysis of 22 sporting federation-banned anabolic agents (or their metabolite markers) and anti-estrogens in urine that are refractory to analysis by gas chromatography-mass spectrometry is presented. In addition, a quantitative method built around World Anti-Doping Agency (WADA) guidelines for the confirmatory analysis of 19-norandrosterone, the primary metabolite of nandrolone with a WADA-specified minimum required performance limit of 1 ng/mL, is included. Hydrolysis of glucuronide conjugates, liquid-liquid extraction, no clean-up derivatization with Girard's Reagent P, and analysis by quadrupole-time-of-flight mass spectrometry provide sensitivity and selectivity well beyond that required by the WADA. |
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Authors:
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Chad R Borges; Nicole Miller; Melinda Shelby; Megan Hansen; Claudia White; Matthew H Slawson; Kim Monti; Dennis J Crouch |
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Publication Detail:
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Type: Journal Article; Validation Studies |
Journal Detail:
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Title: Journal of analytical toxicology Volume: 31 ISSN: 0146-4760 ISO Abbreviation: J Anal Toxicol Publication Date: 2007 Apr |
Date Detail:
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Created Date: 2007-06-20 Completed Date: 2007-07-11 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7705085 Medline TA: J Anal Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 125-31 Citation Subset: IM |
Affiliation:
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Sports Medicine Research and Testing Laboratory, Department of Pharmacology and Toxicology, University of Utah, Salt Lake City, Utah 84108, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anabolic Agents
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urine* Chromatography, High Pressure Liquid* Doping in Sports* Estranes / urine* Estrogen Receptor Modulators / urine* Guidelines as Topic Humans International Agencies Reproducibility of Results Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization* Substance Abuse Detection / methods* Tandem Mass Spectrometry* |
| Chemical | |
Reg. No./Substance:
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0/Anabolic Agents; 0/Estranes; 0/Estrogen Receptor Modulators; 65556-19-6/19-norandrosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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