Document Detail


Analysis of cellular populations in peritoneal effluents of children on CAPD.
MedLine Citation:
PMID:  2582649     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The characterization of cellular populations in peritoneal effluents (PE) of CAPD patients has been the subject of few studies. In order to establish a possible correlation between PE cells and clinical parameters, we studied the immunocompetent cells that are found in uncomplicated patients. In this study we used cytofluorimetric analysis to characterize phenotypically immunocompetent PE cells. We studied 17 children with a mean age of 8.7 +/- 5.3 years, who had been on CAPD for a mean duration of 16 months. The patients never suffered from peritonitis. The effluents were collected after overnight dialysis and centrifuged to concentrate cells. Surface phenotype of PE cells was tested with a panel of monoclonal antibodies. The analysis of 40 PE samples showed that the various cell types were present with different frequencies, suggesting that a dominant phenotype cannot be defined. The ratio between M3 positive cells (monocytic-macrophagic lineage) and T3 positive cells (T lymphocytes) showed a tendency to decrease after initiation of CAPD. T4/T8 ratio in all samples did not differ from that of peripheral lymphocytes. A high frequency of activated T cells (41% +/- 13), defined by the presence of DR antigens on T3+ M3- cells, was seen in PE and confirmed by the high frequency of T cells expressing the IL-2 receptor.
Authors:
M T Valle; M L Degl'Innocenti; P Giordano; A Kunkl; M T Costantini; F Perfumo; F Manca; R Gusmano
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical nephrology     Volume:  32     ISSN:  0301-0430     ISO Abbreviation:  Clin. Nephrol.     Publication Date:  1989 Nov 
Date Detail:
Created Date:  1989-12-28     Completed Date:  1989-12-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0364441     Medline TA:  Clin Nephrol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  235-8     Citation Subset:  IM    
Affiliation:
Immunology Unit, S. Martino Hospital, XIII USL, Genoa, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Child
Child, Preschool
Flow Cytometry / methods
Humans
Kidney Failure, Chronic / therapy*
Macrophages / cytology
Monocytes / cytology
Peritoneal Cavity / pathology*
Peritoneal Dialysis, Continuous Ambulatory*
T-Lymphocytes / cytology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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