| Analysis of cell cycle phases and progression in cultured mammalian cells. | |
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MedLine Citation:
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PMID: 17189856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescence activated cell sorting (FACS) analysis has become a standard tool to analyze cell cycle distributions in populations of cells. These methods require relatively large numbers of cells, and do not provide optimal resolution of the transitions between cell cycle phases. In this report we describe in detail complementary methods that utilize the incorporation of nucleotide analogs combined with microscopic examination. While often more time consuming, these protocols typically require far fewer cells, and allow accurate kinetic assessment of cell cycle progression. We also describe the use of a technique for the synchronization of adherent cells in mitosis by simple mechanical agitation (mitotic shake-off) that eliminates physiological perturbation associated with drug treatments. |
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Authors:
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Christoph Schorl; John M Sedivy |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Review |
Journal Detail:
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Title: Methods (San Diego, Calif.) Volume: 41 ISSN: 1046-2023 ISO Abbreviation: Methods Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2006-12-26 Completed Date: 2007-06-01 Revised Date: 2011-03-09 |
Medline Journal Info:
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Nlm Unique ID: 9426302 Medline TA: Methods Country: United States |
Other Details:
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Languages: eng Pagination: 143-50 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Cell Biology and Biochemistry, and Center for Genomics and Proteomics, Brown University, 70 Ship Street, Providence, RI 02903, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion Cell Cycle / physiology* Cell Line Cell Proliferation Humans Staining and Labeling / methods* |
| Grant Support | |
ID/Acronym/Agency:
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P20 RR15578/RR/NCRR NIH HHS; R01 AG016694-08/AG/NIA NIH HHS; R01 GM041690-18/GM/NIGMS NIH HHS; R01 GM41690/GM/NIGMS NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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