Document Detail


Analysis of causes for formation of chimeras.
MedLine Citation:
PMID:  20045389     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study investigated cDNA chimeras using two closely related members of the rice secretory protein gene family as an example. The chimeras detected in initial cDNA products that were amplified using LA Taq polymerase involved two categories: single-site type and multiple-site type with the frequency being about 20% and 3%, respectively. Further investigation revealed that PCR buffer additives and type of DNA polymerase had a major effect on the formation of chimeras in mixed-template amplification. Heteroduplex repair by microbial DNA repair systems in cDNA cloning was confirmed to produce the chimeras too, but it was not the major source.
Authors:
Xiaobo Zhang; Gang Pan; Xucheng Jin; Qicai Tang; Hong Wang; Jumin Tu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Technical Report     Date:  2010-01-04
Journal Detail:
Title:  Analytical biochemistry     Volume:  400     ISSN:  1096-0309     ISO Abbreviation:  Anal. Biochem.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-22     Completed Date:  2010-06-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  298-300     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Affiliation:
Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Kaixuan Rd 268, Hangzhou 310029, China.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
DNA, Complementary / analysis,  chemistry*
Molecular Sequence Data
Nucleic Acid Heteroduplexes
Oryza sativa / genetics
Plant Proteins / genetics*
Polymerase Chain Reaction
RNA, Plant
Reverse Transcriptase Polymerase Chain Reaction / methods*
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Nucleic Acid Heteroduplexes; 0/Plant Proteins; 0/RNA, Plant

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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