Document Detail


Analysis of cDNA clones encoding sucrose-phosphate synthase in relation to sugar interconversions associated with dehydration in the resurrection plant Craterostigma plantagineum Hochst.
MedLine Citation:
PMID:  9306694     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sucrose-phosphate synthase (SPS) is a key enzyme in the regulation of sucrose metabolism, being responsible for the synthesis of sucrose 6-phosphate from fructose 6-phosphate and uridine 5'-diphosphate-glucose. We report on the isolation and characterization of cDNA clones encoding SPS from Craterostigma plantagineum Hochst., a resurrection plant in which the accumulation of sucrose is considered to play an important role in tolerance to severe protoplastic dehydration. Two distinct classes of cDNAs encoding SPS were isolated from C. plantagineum, and are represented by the clones Cpsps1 and Cpsps2. The transcripts corresponding to both cDNAs decrease to very low levels in dehydrating leaves of C. plantagineum. Only the Cpsps1 transcript occurs in the roots, where it is present at a higher level than in leaves and increases upon dehydration of the plant. Higher enzymatic activities have been determined in protein extracts of dehydrated tissues compared with untreated tissues, which correlates with an increase in protein levels. It is suggested that the overall regulation of SPS is strongly influenced by the changing composition of the cytoplasm in C. plantagineum leaves during the dehydration-rehydration cycle.
Authors:
J Ingram; J W Chandler; L Gallagher; F Salamini; D Bartels
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  115     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-10-29     Completed Date:  1997-10-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  113-21     Citation Subset:  IM    
Affiliation:
Max Planck Institut für Züchtungsforschung, Köln, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Y11795;  Y11821
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Base Sequence
Carbohydrate Metabolism
DNA Primers / genetics
DNA, Complementary / genetics*
DNA, Plant / genetics*
Dehydration / enzymology,  genetics,  metabolism
Gene Expression Regulation, Plant
Genes, Plant
Glucosyltransferases / genetics*,  metabolism
Molecular Sequence Data
Plants / enzymology*,  genetics*,  metabolism
Sequence Homology, Amino Acid
Water / metabolism
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Complementary; 0/DNA, Plant; 7732-18-5/Water; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.14/sucrose-phosphate synthase
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