| Analysis of cDNA clones encoding sucrose-phosphate synthase in relation to sugar interconversions associated with dehydration in the resurrection plant Craterostigma plantagineum Hochst. | |
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MedLine Citation:
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PMID: 9306694 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sucrose-phosphate synthase (SPS) is a key enzyme in the regulation of sucrose metabolism, being responsible for the synthesis of sucrose 6-phosphate from fructose 6-phosphate and uridine 5'-diphosphate-glucose. We report on the isolation and characterization of cDNA clones encoding SPS from Craterostigma plantagineum Hochst., a resurrection plant in which the accumulation of sucrose is considered to play an important role in tolerance to severe protoplastic dehydration. Two distinct classes of cDNAs encoding SPS were isolated from C. plantagineum, and are represented by the clones Cpsps1 and Cpsps2. The transcripts corresponding to both cDNAs decrease to very low levels in dehydrating leaves of C. plantagineum. Only the Cpsps1 transcript occurs in the roots, where it is present at a higher level than in leaves and increases upon dehydration of the plant. Higher enzymatic activities have been determined in protein extracts of dehydrated tissues compared with untreated tissues, which correlates with an increase in protein levels. It is suggested that the overall regulation of SPS is strongly influenced by the changing composition of the cytoplasm in C. plantagineum leaves during the dehydration-rehydration cycle. |
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Authors:
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J Ingram; J W Chandler; L Gallagher; F Salamini; D Bartels |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Plant physiology Volume: 115 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 1997 Sep |
Date Detail:
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Created Date: 1997-10-29 Completed Date: 1997-10-29 Revised Date: 2010-09-14 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 113-21 Citation Subset: IM |
Affiliation:
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Max Planck Institut für Züchtungsforschung, Köln, Germany. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/Y11795; Y11821 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Base Sequence Carbohydrate Metabolism DNA Primers / genetics DNA, Complementary / genetics* DNA, Plant / genetics* Dehydration / enzymology, genetics, metabolism Gene Expression Regulation, Plant Genes, Plant Glucosyltransferases / genetics*, metabolism Molecular Sequence Data Plants / enzymology*, genetics*, metabolism Sequence Homology, Amino Acid Water / metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Complementary; 0/DNA, Plant; 7732-18-5/Water; EC 2.4.1.-/Glucosyltransferases; EC 2.4.1.14/sucrose-phosphate synthase |
| Comments/Corrections | |
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