Document Detail


Analysis by flow cytometry of surface-exposed epitopes of outer membrane protein F of Pseudomonas aeruginosa.
MedLine Citation:
PMID:  8776855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antisera were produced in mice immunized with 18 synthetic peptide conjugates representing various regions throughout the length of the outer membrane protein F molecule of Pseudomonas aeruginosa and analysed by flow cytometry to identify those antisera capable of binding to the surface of whole cells of P. aeruginosa. Antibodies to peptides 9, 18, 10, and 4 were significantly cell-surface reactive. The maximum median percentage of antibody-binding cells in this assay was 36.6%. Over six different determinations, peptide 9 antisera binding to the cells ranged from 16.9 to 57.0% of the cell population. We propose that the surface accessibility of protein F epitopes varies during the cell cycle.
Authors:
E E Hughes; J M Matthews-Greer; H E Gilleland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  42     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1996 Aug 
Date Detail:
Created Date:  1997-03-20     Completed Date:  1997-03-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  859-62     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, Louisiana State University Medical Center, School of Medicine in Shreveport 71130-3932, USA.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Bacterial
Bacterial Outer Membrane Proteins / immunology*
Epitopes*
Flow Cytometry / methods*
Oligopeptides / immunology
Pseudomonas aeruginosa / immunology*
Surface Properties
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Bacterial Outer Membrane Proteins; 0/Epitopes; 0/Oligopeptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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