Document Detail

Analysis of blood plasma proteins in patients with Alzheimer's disease by two-dimensional electrophoresis, sequence homology and immunodetection.
MedLine Citation:
PMID:  10870969     Owner:  NLM     Status:  MEDLINE    
Blood plasma proteins of patients with Alzheimer's disease (AD; senile dementia) and non-AD-type dementia were resolved by two-dimensional electrophoresis and identified by migration position in the electrophoresis pattern, sequence homology, and immunodetection by using antibodies. For the control experiments, blood plasma proteins of a healthy young individual and non-dementia patients were examined in a manner similar to that of the plasma samples of AD patients. In the plasma sample of the healthy young individual, more than 350 spots of silver-stained proteins were observed and among these spots, 73 spots were identified. Blood plasma proteins of the AD and non-AD-type dementia patients were compared with those of the control and non-dementia patients. In the blood plasma samples of five AD patients, three patients had apolipoprotein E4, and another patient showed apolipoprotein L and complement factor H. For the AD-related proteins apolipoprotein E, tau-1, and presenilin 2, proteins were examined by immunostaining with antibodies, in both AD and non-AD patients. Among the three samples of non-AD-type dementia patients, one was distinguishable by amyloid A proteins, and the other by haptoglobin isoforms.
I Ueno; T Sakai; M Yamaoka; R Yoshida; A Tsugita
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  21     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-11-03     Completed Date:  2000-11-03     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  1832-45     Citation Subset:  IM    
Research Institute for Biosciences, Science University of Tokyo, Noda, Japan.
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MeSH Terms
Aged, 80 and over
Alzheimer Disease / blood*
Amino Acid Sequence
Blood Proteins / analysis*
Blotting, Western / methods
Electrophoresis, Gel, Two-Dimensional / methods
Molecular Sequence Data
Sequence Homology, Amino Acid
Staining and Labeling / methods
Reg. No./Substance:
0/Blood Proteins

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