Document Detail


Analysis of blood plasma proteins in patients with Alzheimer's disease by two-dimensional electrophoresis, sequence homology and immunodetection.
MedLine Citation:
PMID:  10870969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Blood plasma proteins of patients with Alzheimer's disease (AD; senile dementia) and non-AD-type dementia were resolved by two-dimensional electrophoresis and identified by migration position in the electrophoresis pattern, sequence homology, and immunodetection by using antibodies. For the control experiments, blood plasma proteins of a healthy young individual and non-dementia patients were examined in a manner similar to that of the plasma samples of AD patients. In the plasma sample of the healthy young individual, more than 350 spots of silver-stained proteins were observed and among these spots, 73 spots were identified. Blood plasma proteins of the AD and non-AD-type dementia patients were compared with those of the control and non-dementia patients. In the blood plasma samples of five AD patients, three patients had apolipoprotein E4, and another patient showed apolipoprotein L and complement factor H. For the AD-related proteins apolipoprotein E, tau-1, and presenilin 2, proteins were examined by immunostaining with antibodies, in both AD and non-AD patients. Among the three samples of non-AD-type dementia patients, one was distinguishable by amyloid A proteins, and the other by haptoglobin isoforms.
Authors:
I Ueno; T Sakai; M Yamaoka; R Yoshida; A Tsugita
Related Documents :
2094379 - Confusional symptomatology distinguishes early- and late-onset alzheimer's disease.
1491839 - Cognition and 99tcm-hmpao spect in parkinson's disease.
18558299 - Hypoalbuminemia and not hyperhomocysteinemia as a risk factor for dementia in hemodialy...
9730709 - 99mtc-labelled red blood cell scintigraphy for localization of gastrointestinal bleedin...
10932269 - Unilateral spatial neglect in ad: significance of line bisection performance.
17994279 - Regional analysis of spontaneous meg rhythms in patients with alzheimer's disease using...
3364189 - Coracoid process transfer for acromioclavicular dislocation.
8532109 - Circadian locomotor activity and core-body temperature rhythms in alzheimer's disease.
3960279 - Spontaneous remission of cerebral palsy.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Electrophoresis     Volume:  21     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-11-03     Completed Date:  2000-11-03     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  1832-45     Citation Subset:  IM    
Affiliation:
Research Institute for Biosciences, Science University of Tokyo, Noda, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Alzheimer Disease / blood*
Amino Acid Sequence
Blood Proteins / analysis*
Blotting, Western / methods
Electrophoresis, Gel, Two-Dimensional / methods
Female
Humans
Male
Molecular Sequence Data
Sequence Homology, Amino Acid
Staining and Labeling / methods
Chemical
Reg. No./Substance:
0/Blood Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Comprehensive analyses of prostate gene expression: convergence of expressed sequence tag databases,...
Next Document:  Alteration of protein composition in mouse thymocytes by signals through T-cell receptor.