Document Detail


Analysis of the bacterial communities present in lungs of patients with cystic fibrosis from American and British centers.
MedLine Citation:
PMID:  21068277     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to determine whether geographical differences impact the composition of bacterial communities present in the airways of cystic fibrosis (CF) patients attending CF centers in the United States or United Kingdom. Thirty-eight patients were matched on the basis of clinical parameters into 19 pairs comprised of one U.S. and one United Kingdom patient. Analysis was performed to determine what, if any, bacterial correlates could be identified. Two culture-independent strategies were used: terminal restriction fragment length polymorphism (T-RFLP) profiling and 16S rRNA clone sequencing. Overall, 73 different terminal restriction fragment lengths were detected, ranging from 2 to 10 for U.S. and 2 to 15 for United Kingdom patients. The statistical analysis of T-RFLP data indicated that patient pairing was successful and revealed substantial transatlantic similarities in the bacterial communities. A small number of bands was present in the vast majority of patients in both locations, indicating that these are species common to the CF lung. Clone sequence analysis also revealed that a number of species not traditionally associated with the CF lung were present in both sample groups. The species number per sample was similar, but differences in species presence were observed between sample groups. Cluster analysis revealed geographical differences in bacterial presence and relative species abundance. Overall, the U.S. samples showed tighter clustering with each other compared to that of United Kingdom samples, which may reflect the lower diversity detected in the U.S. sample group. The impact of cross-infection and biogeography is considered, and the implications for treating CF lung infections also are discussed.
Authors:
Franziska A Stressmann; Geraint B Rogers; Erich R Klem; Andrew K Lilley; Scott H Donaldson; Thomas W Daniels; Mary P Carroll; Nilesh Patel; Benjamin Forbes; Richard C Boucher; Matthew C Wolfgang; Kenneth D Bruce
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-11-10
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  49     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-03     Completed Date:  2011-04-11     Revised Date:  2013-07-03    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  281-91     Citation Subset:  IM    
Affiliation:
Molecular Microbiology Research Laboratory, Pharmaceutical Science Research Division, King's College London, 150 Stamford Street, Franklin-Wilkins Building, London, SE1 9NH, United Kingdom.
Data Bank Information
Bank Name/Acc. No.:
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FN826731;  FN826732;  FN826733;  FN826734;  FN826735;  FN826736;  FN826737;  FN826738;  FN826739;  FN826740;  FN826741;  FN826742;  FN826743;  FN826744;  FN826745;  FN826746;  FN826747;  FN826748;  FN826749;  FN826750;  FN826751;  FN826752;  FN826753;  FN826754;  FN826755;  FN826756;  FN826757;  FN826758;  FN826759;  FN826760;  FN826761;  FN826762;  FN826763;  FN826764;  FN826765;  FN826766;  FN826767;  FN826768;  FN826769;  FN826770;  FN826771;  FN826772;  FN826773;  FN826774;  FN826775;  FN826776;  FN826777;  FN826778;  FN826779;  FN826780;  FN826781;  FN826782;  FN826783
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Bacteria / classification*,  genetics,  isolation & purification*
Biodiversity*
Cluster Analysis
Cystic Fibrosis / microbiology*
DNA, Bacterial / chemistry,  genetics
DNA, Ribosomal / chemistry,  genetics
Female
Great Britain
Humans
Lung / microbiology*
Male
Middle Aged
Molecular Sequence Data
Phylogeny
Polymorphism, Restriction Fragment Length
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
United States
Young Adult
Grant Support
ID/Acronym/Agency:
HL092964/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S
Comments/Corrections

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