| Analysis of apoptosis induced by Caprine Herpesvirus 1 in vitro. | |
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MedLine Citation:
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PMID: 19619589 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It is known that Caprine Herpesvirus 1 (CpHV-1) causes apoptosis in mitogen-stimulated as well as not stimulated caprine peripheral blood mononuclear cells (PBMC). Initial experiments in Madin Darby bovine kidney (MDBK) cells revealed that CpHV-1 infection induced apoptotic features like chromatin condensation and DNA laddering. Thus, to characterize in more detail this apoptotic process, activation of caspase-8, -9 and -3 in MDBK cells CpHV-1 infected was investigated and demonstrated. In addition, CpHV-1 infection resulted in disruption of mitochondrial membrane potential, cytochrome c release and alterations in the pro- and anti-apoptotic proteins of Bcl-2 family. Proteolytic cleavage of poly(ADP-ribose) polymerases (PARP), confirming the activation of downstream caspases, was also observed. Our data indicated that a "cross-talk" between the death-receptor (extrinsic) pathway and the mitochondrial (intrinsic) pathway occurred in CpHV-1-induced apoptosis in vitro. |
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Authors:
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M Longo; F Fiorito; G Marfè; S Montagnaro; G Pisanelli; L De Martino; G Iovane; U Pagnini |
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Publication Detail:
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Type: Journal Article Date: 2009-07-18 |
Journal Detail:
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Title: Virus research Volume: 145 ISSN: 1872-7492 ISO Abbreviation: Virus Res. Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-09-28 Completed Date: 2009-11-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8410979 Medline TA: Virus Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 227-35 Citation Subset: IM |
Affiliation:
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Department of Pathology and Animal Health, Infectious Diseases, Faculty of Veterinary Medicine, University of Naples Federico II, Via F. Delpino 1, 80137 Naples, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Caspase 3 / biosynthesis Caspase 8 / biosynthesis Caspase 9 / biosynthesis Cattle Cell Line Cytochromes c / metabolism Membrane Potential, Mitochondrial Poly(ADP-ribose) Polymerases / metabolism Proto-Oncogene Proteins c-bcl-2 / metabolism Up-Regulation Varicellovirus / pathogenicity* |
| Chemical | |
Reg. No./Substance:
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0/Proto-Oncogene Proteins c-bcl-2; 9007-43-6/Cytochromes c; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 8; EC 3.4.22.-/Caspase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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